ArticleNature2019
Comprehensive single-cell transcriptome lineages of a proto-vertebrate.
Article in Nature, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 137 papers.
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Who cites it
137 citing papers in PubMed.
- Spatial transcriptomics ofiScience · 2026Article
- Lifelong Invertebrate Chordate Central Nervous System Regeneration is Driven by a Migrating Pool of Conserved Neural Stem Cells program.bioRxiv : the preprint server for biology · 2026Article
- Similarities betweeneNeuro · 2026Article
- Reassessing forebrain organization: recent evidence challenges the prosomere model.Current opinion in genetics & development · 2026Review
- A thyroid hormone-mediated opsin switch initiates metamorphosis in a proto-vertebrate.Science advances · 2026Article
- Reference-free discovery with barcoded single-cell sequencing.Nature biotechnology · 2026Article
- Single-cell, clonal and spatial atlases of cranial placodes illuminate their specification and evolution.bioRxiv : the preprint server for biology · 2026Article
- Neural signaling contributes to heart formation and growth in the invertebrate chordate, Ciona robusta.PLoS biology · 2026Article
- Article
- Cell numbers contribute to cell fate during Ciona cardiopharyngeal mesoderm specification.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- DOO: integrated multi-omics resources for deep ocean organisms.Nucleic acids research · 2026Article
- Tunicates have a complex, highly dynamic TNF superfamily.PloS one · 2026Article
- Marine Model Organisms for Mechanobiology Studies.Results and problems in cell differentiation · 2026Review
- Proteome analysis refines molecular processes underlying metamorphosis in the ascidian Ciona intestinalis.PloS one · 2026Article
- Divergence of immune cell types in chordate blood.Current biology : CB · 2025Article
- Pair-rule-like transcription patterns during neural tube closure in a proto-vertebrate.Development (Cambridge, England) · 2025Article
- A mannitol-based buffer improves single-cell RNA sequencing of high-salt marine cells.BMC genomics · 2025Article
- The tunicate Ciona.Nature methods · 2025Article
- Cell cycle-driven transcriptome maturation confers multilineage competence to cardiopharyngeal progenitors.The EMBO journal · 2025Article
- Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: insights into tissue specialization and blastogenesis.Development (Cambridge, England) · 2025Article
77 more citing papers are in PubMed but not listed here.
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10 authors.
Funding
Abstract
Ascidian embryos highlight the importance of cell lineages in animal development. As simple proto-vertebrates, they also provide insights into the evolutionary origins of cell types such as cranial placodes and neural crest cells. Here we have determined single-cell transcriptomes for more than 90,000 cells that span the entirety of development-from the onset of gastrulation to swimming tadpoles-in Ciona intestinalis. Owing to the small numbers of cells in ascidian embryos, this represents an average of over 12-fold coverage for every cell at every stage of development. We used single-cell transcriptome trajectories to construct virtual cell-lineage maps and provisional gene networks for 41 neural subtypes that comprise the larval nervous system. We summarize several applications of these datasets, including annotating the synaptome of swimming tadpoles and tracing the evolutionary origin of cell types such as the vertebrate telencephalon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.