ArticleArteriosclerosis, thrombosis, and vascular biology2019
Turbulent Flow Promotes Cleavage of VWF (von Willebrand Factor) by ADAMTS13 (A Disintegrin and Metalloproteinase With a Thrombospondin Type-1 Motif, Member 13).
Article in Arteriosclerosis, thrombosis, and vascular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 47 citations in OpenAlex.
- Pulsatility as a Potential Regulator of Cardiovascular Biology: Molecular, Cellular, and Hemodynamic Remodeling During Continuous-Flow Left Ventricular Assist Device Support and Following Heart Transplantation.International journal of molecular sciences · 2026Review
- Numerical Analyses of Blood Pumps Under Realistic Operating Conditions.ASAIO journal (American Society for Artificial Internal Organs : 1992) · 2026Article
- Vorticity-Facilitated Platelet Aggregation: a High Expansion-Ratio Stenotic Microfluidic Platform Unravels the Role of Complex Flow Dynamics in Arterial Thrombosis.Advanced healthcare materials · 2025Article
- Platelet-Related Biomarkers and Efficacy of Antiplatelet Therapy in Patients with Aortic Stenosis and Coronary Artery Disease.International journal of molecular sciences · 2025Article
- Understanding the role of vascular stretch on modulation of VWF and ANGPT-2 in continuous flow left ventricular assist device (CF-VAD) patients.Lab on a chip · 2025Article
- Recommendations for defining disturbed flow as laminar, transitional, or turbulent in assays of hemostasis and thrombosis: communication from the ISTH SSC Subcommittee on Biorheology.Journal of thrombosis and haemostasis : JTH · 2025Review
- Influence of Aortic Valve Stenosis and Wall Shear Stress on Platelets Function.Journal of clinical medicine · 2023Review
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- Turbulence in surgical suction heads as detected by MRI.The journal of extra-corporeal technology · 2023Article
- Loss of pulsatility with continuous-flow left ventricular assist devices and the significance of the arterial endothelium in von-Willebrand factor production and degradation.Artificial organs · 2023Review
- Article
- The effect of recombinant versus plasma-derived von Willebrand factor on prolonged PFA closure times in ECMO patients with acquired von Willebrand syndrome - an observational study.Thrombosis journal · 2023Article
- Conformation of von Willebrand factor in shear flow revealed with stroboscopic single-molecule imaging.Blood · 2022Article
- Tip-end fusion of a rod-shaped secretory organelle.Cellular and molecular life sciences : CMLS · 2022Article
- The Intriguing Connections between von Willebrand Factor, ADAMTS13 and Cancer.Healthcare (Basel, Switzerland) · 2022Review
- Distribution and history of extensional stresses on vWF surrogate molecules in turbulent flow.Scientific reports · 2022Article
- Article
- A Continuum Model for the Unfolding of von Willebrand Factor.Annals of biomedical engineering · 2021Article
- Physical forces regulating hemostasis and thrombosis: Vessels, cells, and molecules in illustrated review.Research and practice in thrombosis and haemostasis · 2021Review
- Shear-mediated platelet activation in the free flow II: Evolving mechanobiological mechanisms reveal an identifiable signature of activation and a bi-directional platelet dyscrasia with thrombotic and bleeding features.Journal of biomechanics · 2021Review
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8 authors at 3 institutions in 1 country.
Funding
Abstract
Objective- Acquired von Willebrand syndrome is defined by excessive cleavage of the VWF (von Willebrand Factor) and is associated with impaired primary hemostasis and severe bleeding. It often develops when blood is exposed to nonphysiological flow such as in aortic stenosis or mechanical circulatory support. We evaluated the role of laminar, transitional, and turbulent flow on VWF cleavage and the effects on VWF function. Approach and Results- We used a vane rheometer to generate laminar, transitional, and turbulent flow and evaluate the effect of each on VWF cleavage in the presence of ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type-1 motif, member 13). We performed functional assays to evaluate the effect of these flows on VWF structure and function. Computational fluid dynamics was used to estimate the flow fields and forces within the vane rheometer under each flow condition. Turbulent flow is required for excessive cleavage of VWF in an ADAMTS13-dependent manner. The assay was repeated with whole blood, and the turbulent flow had the same effect. Our computational fluid dynamics results show that under turbulent conditions, the Kolmogorov scale approaches the size of VWF. Finally, cleavage of VWF in this study has functional consequences under flow as the resulting VWF has decreased ability to bind platelets and collagen. Conclusions- Turbulent flow mediates VWF cleavage in the presence of ADAMTS13, decreasing the ability of VWF to sustain platelet adhesion. These findings impact the design of mechanical circulatory support devices and are relevant to pathological environments where turbulence is added to circulation.
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