Evidence map›Paper›PMID 31291257›Full record

ArticlePloS one2019

CD147 mediates intrahepatic leukocyte aggregation and determines the extent of liver injury.

Christine Yee, Nathan M Main, Alexandra Terry, Igor Stevanovski, Annette Maczurek, Alison J Morgan, Sarah Calabro, Alison J Potter, Tina L Iemma, David G Bowen and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Surfaceome Profiling Identifies Basigin-Chaperoned Protein Clients.Chembiochem : a European journal of chemical biology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Christine YeeCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Nathan M MainGastroenterology and Liver Laboratory, Ingham Institute for Applied Medical Research, Liverpool, NSW, Australia.ORCID 0000-0003-0966-0745
Alexandra TerryCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Igor StevanovskiGastroenterology and Liver Laboratory, Ingham Institute for Applied Medical Research, Liverpool, NSW, Australia.
Annette MaczurekCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Alison J MorganCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Sarah CalabroCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Alison J PotterCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Tina L IemmaCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
David G BowenCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Golo AhlenstielWestern Sydney School of Medicine, Blacktown Hospital, Blacktown, NSW, Australia.
Fiona J WarnerCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Geoffrey W McCaughanCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
Susan V McLennanDepartment of Endocrinology, Department of Medicine and Bosch Institute, Royal Prince Alfred Hospital, The University of Sydney, NSW, Australia.
Nicholas A ShackelCentenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, NSW, Australia.
The University of Sydney · AUIngham Institute · AUBlacktown & Mount Druitt Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChronic inflammation is the driver of liver injury and results in progressive fibrosis and eventual cirrhosis with consequences including both liver failure and liver cancer. We have previously described increased expression of the highly multifunctional glycoprotein CD147 in liver injury. This work describes a novel role of CD147 in liver inflammation and the importance of leukocyte aggregates in determining the extent of liver injury.

methodsNon-diseased, progressive injury, and cirrhotic liver from humans and mice were examined using a mAb targeting CD147. Inflammatory cell subsets were assessed by multiparameter flow cytometry.

resultsIn liver injury, we observe abundant, intrahepatic leukocyte clusters defined as ≥5 adjacent CD45+ cells which we have termed "leukocyte aggregates". We have shown that these leukocyte aggregates have a significant effect in determining the extent of liver injury. If CD147 is blocked in vivo, these leukocyte aggregates diminish in size and number, together with a marked significant reduction in liver injury including fibrosis. This is accompanied by no change in overall intrahepatic leukocyte numbers. Further, blocking of aggregation formation occurs prior to an appreciable increase in inflammatory markers or fibrosis. Additionally, there were no observed, "off-target" or unpredicted effects in targeting CD147.

conclusionCD147 mediates leukocyte aggregation which is associated with the development of liver injury. This is not a secondary effect, but a cause of injury as aggregate formation proceeds other markers of injury. Leukocyte aggregation has been previously described in inflammation dating back over many decades. Here we demonstrate that leukocyte aggregates determine the extent of liver injury.

Indexed as

AnimalsBasiginCell AggregationHepatocytesHumansLeukocytesLiverLiver CirrhosisLiver Cirrhosis, ExperimentalMaleMiceMice, Inbred BALB CMice, Inbred C57BLUp-RegulationBasiginBSG protein, humanBsg protein, mouse

Identifiers

PMID31291257
PMCPMC6619953
OpenAlexW2960739315

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.