ArticleScientific reports2019
Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Reduced CH25H expression defines a high membrane fluidity, trogocytosis active state in colon cancer stem cells.Frontiers in cell and developmental biology · 2026Article
- Alternatives to animal models in gastroenterology and hepatology research.Frontiers in pharmacology · 2026Review
- Emerging advances in intestinal models for in vitro preclinical research.American journal of physiology. Gastrointestinal and liver physiology · 2025Review
- Human intestinal enteroids: Nonclinical applications for predicting oral drug disposition, toxicity, and efficacy.Pharmacology & therapeutics · 2025Review
- Green/red fluorescent protein disrupting drugs for real-time permeability tracking in three-dimensional tumor spheroids.Bioengineering & translational medicine · 2025Article
- Antiviral Effect of Melatonin on Caco-2 Cell Organoid Culture: Trick or Treat?International journal of molecular sciences · 2024Article
- Intestinal organ chips for disease modelling and personalized medicine.Nature reviews. Gastroenterology & hepatology · 2024Review
- Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning.Arthritis research & therapy · 2024Article
- The potential of micro- and nanoplastics to exacerbate the health impacts and global burden of non-communicable diseases.Cell reports. Medicine · 2024Review
- A comprehensive review on 3D tissue models: Biofabrication technologies and preclinical applications.Biomaterials · 2024Review
- Design and Development of Extracellular Matrix Protein-Based Microcapsules as Tools for Bacteria Investigation.Advanced healthcare materials · 2023Article
- Intestinal Cell Differentiation and Phenotype in 2D and 3D Cell Culture Models.Methods in molecular biology (Clifton, N.J.) · 2023Article
- An integratedInternational journal of bioprinting · 2023Article
- Morphological and molecular characteristics of spheroid formation in HT-29 and Caco-2 colorectal cancer cell lines.Cancer cell international · 2021Article
- Networks of High Aspect Ratio Particles to Direct Colloidal Assembly Dynamics and Cellular Interactions.Advanced functional materials · 2020Article
- Micro and nanoscale technologies in oral drug delivery.Advanced drug delivery reviews · 2020Review
- Oral administration of M13-loaded nanoliposomes is safe and effective to treat colitis-associated cancer in mice.Expert opinion on drug deliveryArticle
- Enhancing pre-clinical research with simplified intestinal cell line models.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In vitro models of the small intestine are crucial tools for the prediction of drug absorption. The Caco-2 monolayer transwell model has been widely employed to assess drug absorption across the intestine. However, it is now well-established that 3D in vitro models capture tissue-specific architecture and interactions with the extracellular matrix and therefore better recapitulate the complex in vivo environment. However, these models need to be characterized for barrier properties and changes in gene expression and transporter function. Here, we report that geometrically controlled self-assembling multicellular intestinal Caco-2 spheroids cultured using Sacrificial Micromolding display reproducible intestinal features and functions that are more representative of the in vivo small intestine than the widely used 2D transwell model. We show that Caco-2 cell maturation and differentiation into the intestinal epithelial phenotype occur faster in spheroids and that they are viable for a longer period of time. Finally, we were able to invert the polarity of the spheroids by culturing them around Matrigel beads allowing superficial access to the apical membrane and making the model more physiological. This robust and reproducible in vitro intestinal model could serve as a valuable system to expedite drug screening as well as to study intestinal transporter function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.