ArticleThe ISME journal2019
Patterns of protist diversity associated with raw sewage in New York City.
Article in The ISME journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Global prevalence of potentially pathogenic free-living amoebae in sewage and sewage-related environments-systematic review with meta-analysis.Parasitology research · 2024Pooled it
- Deep metatranscriptomic sequencing data of wastewater from Los Angeles, USA, 2023-2024.Scientific data · 2025Article
- Synergistic role of Trichormus variabilis and zeolites in three-layer culturing system for modulating the wastewater effluent community.Scientific reports · 2025Article
- Unravelling the temporal dynamics of community functions in protists induced by treated wastewater exposure using metatranscriptomics.Scientific reports · 2025Article
- Cross-Cutting Approach for the Characterization of Microbial Emerging Hazards in Agriculture Settings from Circular Economy-Driven Wastewater Streams.Environment & health (Washington, D.C.) · 2024Review
- Molecular Prevalence and Subtypes Distribution ofTropical medicine and infectious disease · 2024Review
- Diabetes and gut microbiome.Frontiers in microbiology · 2024Review
- PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats.Scientific reports · 2023Article
- PacBio next-generation sequencing uncovers Apicomplexa diversity in different habitats.Scientific reports · 2023Article
- Metagenomic Profiling of Human Protozoan Parasites in Wastewater and Hospital Effluents.Journal of clinical practice and research · 2023Article
- The Effect of Protozoa Indigenous to Lakewater and Wastewater on Decay of Fecal Indicator Bacteria and Coliphage.Pathogens (Basel, Switzerland) · 2023Article
- Toward an intensive understanding of sewer sediment prokaryotic community assembly and function.Frontiers in microbiology · 2023Article
- Diagnostic Methods of Common Intestinal Protozoa: Current and Future Immunological and Molecular Methods.Tropical medicine and infectious disease · 2022Review
- MicrobiomeCensus estimates human population sizes from wastewater samples based on inter-individual variability in gut microbiomes.PLoS computational biology · 2022Article
- A microbiome study reveals the potential relationship between the bacterial diversity of a gymnastics hall and human health.Scientific reports · 2022Article
- Microeukaryotic gut parasites in wastewater treatment plants: diversity, activity, and removal.Microbiome · 2022Article
- Does filter pore size introduce bias in DNA sequence-based plankton community studies?Frontiers in microbiology · 2022Article
- Article
- Initial Mapping of the New York City Wastewater Virome.mSystems · 2020Article
- Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage.mSystems · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protists are ubiquitous components of terrestrial and aquatic environments, as well as animal and human microbiomes. Despite this, little is known about protists in urban environments. The ~7400-mile sewer system of New York City (NYC) collects human waste from ~8 million human inhabitants as well as from animals, street runoff, and groundwater, providing an ideal system to study these microbes. We used 18S rRNA amplicon sequencing and shotgun metagenomic sequencing to profile raw sewage microbial communities. Raw sewage samples were collected over a 12-month period from 14 treatment plants of the five NYC boroughs, and compared with samples from other environments including soil, stormwater, and sediment. Sewage contained a diverse protist community dominated by free-living clades, and communities were highly differentiated across environments. Seasonal differences in protist composition were observed; however, network analysis and functional profiling demonstrated that sewage communities were robust and functionally consistent. Protists typically associated with human and animal guts or feces were frequently detected. Abundance of these parasites varied significantly both spatially and temporally, suggesting that spikes could reflect trends in the source population. This underscores sewage as a valuable model system for monitoring patterns in urban microbes and provides a baseline protist metagenome of NYC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.