ArticleScientific reports2019
Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Development of a semi-endogenous PRT-based expression system for Methylotuvimicrobium alcaliphilum and its application to enhance 3-hydroxybutyric acid production from methanol.Journal of biological engineering · 2025Review
- The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives.Plant communications · 2025Review
- A new tool for engineering Phaeodactylum tricornutum: the METE promoter drives both high expression and BThe Plant journal : for cell and molecular biology · 2025Article
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Characterization of the VOC Promoter That Is Active Under Low-Salinity Conditions in the DiatomMarine drugs · 2025Article
- In Vitro Culture, Genetic Transformation and the Production of Biopharmaceuticals in Microalgae.International journal of molecular sciences · 2025Review
- In vivo thrombin activity in the diatom Phaeodactylum tricornutum: biotechnological insights.Applied microbiology and biotechnology · 2024Article
- Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid.The Plant cell · 2024Article
- Comparative RNA-Seq of TenMarine drugs · 2024Article
- Validating a Promoter Library for Application in Plasmid-Based Diatom Genetic Engineering.ACS synthetic biology · 2023Article
- Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics.Scientific reports · 2022Article
- Phytate as a Phosphorus Nutrient with Impacts on Iron Stress-Related Gene Expression for Phytoplankton: Insights from the DiatomApplied and environmental microbiology · 2022Article
- Comparative Proteomic Analysis of the DiatomFrontiers in plant science · 2022Article
- Telomere-to-telomere genome assembly ofPeerJ · 2022Article
- Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum.MicrobiologyOpen · 2021Article
- Stramenopile microalgae as "green biofactories" for recombinant protein production.World journal of microbiology & biotechnology · 2021Review
- Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes ofFrontiers in plant science · 2021Article
- Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.Scientific reports · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Mobilization and Cellular Distribution of Phosphate in the DiatomFrontiers in plant science · 2020Article
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Authors and funding
5 authors.
Funding
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Abstract
Although diatoms have been extensively studied as bioreactors, only a limited number of efficient gene promoters are available. Therefore, the development of new endogenous promoters is important for the heterologous production of a variety of recombinant proteins. Herein, we identified the most abundant secreted protein in Phaeodactylum tricornutum, designated 'highly abundant secreted protein 1' (HASP1), and characterised the activities of its promoter and signal peptide using green fluorescent protein (GFP) as a reporter. The HASP1 promoter strongly drove GFP expression during all growth phases of P. tricornutum in culture, in contrast to the commonly used fcpA promoter, which is less active during the stationary phase. The HASP1 signal peptide was also sufficient for facilitating efficient secretion of GFP by P. tricornutum. Our findings suggest that both the promoter and the signal peptide of HASP1 can be utilized as novel tools for the overexpression and secretion of recombinant proteins in P. tricornutum.
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