ArticleScientific reports2019
Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
- Autoinducer 2 as a universal language in microbial consortia: decoding molecular mechanisms, ecological impacts, and application.Gut microbes · 2026Review
- LuxS/AI-2 regulates phoP/phoQ by a non-canonical mechanism to enhance acid stress survival in Salmonella Typhimurium.PLoS pathogens · 2026Article
- Comparative genomic analysis ofMicrobial genomics · 2026Article
- The Ambivalent Nature ofToxins · 2025Review
- Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure.NPJ biofilms and microbiomes · 2025Article
- Community-AcquiredInternational journal of molecular sciences · 2025Review
- Enhancing biofilm disruption and bactericidal efficiency using vancomycin-loaded microbubbles in sonodynamic therapy.JAC-antimicrobial resistance · 2025Article
- Review
- In-depth characterization of accessory gene regulator loci and associated virulence factors inCurrent research in microbial sciences · 2025Article
- Targeting quorum sensing for manipulation of commensal microbiota.BMC biotechnology · 2024Review
- Understanding Quorum-Sensing and Biofilm Forming in Anaerobic Bacterial Communities.International journal of molecular sciences · 2024Review
- Challenges and opportunities of phage therapy forApplied and environmental microbiology · 2024Review
- The current riboswitch landscape inMicrobiology (Reading, England) · 2024Review
- Genetic diversity, biofilm formation, and Vancomycin resistance of clinical Clostridium innocuum isolates.BMC microbiology · 2024Article
- Environmental and Nutritional Parameters Modulating Genetic Expression for Virulence Factors ofAntibiotics (Basel, Switzerland) · 2024Review
- Impact of LuxS on virulence and pathogenicity inInfection and immunity · 2024Article
- Membrane Vesicles of Clostridioides difficile and Other Clostridial Species.Advances in experimental medicine and biology · 2024Article
- Clostridioides difficile Biofilm.Advances in experimental medicine and biology · 2024Article
- Removal of mobile genetic elements from the genome ofFrontiers in microbiology · 2024Article
- Review of the Impact of Biofilm Formation on RecurrentMicroorganisms · 2023Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The anaerobic gut pathogen, Clostridioides difficile, forms adherent biofilms that may play an important role in recurrent C. difficile infections. The mechanisms underlying C. difficile community formation and inter-bacterial interactions are nevertheless poorly understood. C. difficile produces AI-2, a quorum sensing molecule that modulates biofilm formation across many bacterial species. We found that a strain defective in LuxS, the enzyme that mediates AI-2 production, is defective in biofilm development in vitro. Transcriptomic analyses of biofilms formed by wild type (WT) and luxS mutant (luxS) strains revealed a downregulation of prophage loci in the luxS mutant biofilms compared to the WT. Detection of phages and eDNA within biofilms may suggest that DNA release by phage-mediated cell lysis contributes to C. difficile biofilm formation. In order to understand if LuxS mediates C. difficile crosstalk with other gut species, C. difficile interactions with a common gut bacterium, Bacteroides fragilis, were studied. We demonstrate that C. difficile growth is significantly reduced when co-cultured with B. fragilis in mixed biofilms. Interestingly, the absence of C. difficile LuxS alleviates the B. fragilis-mediated growth inhibition. Dual species RNA-sequencing analyses from single and mixed biofilms revealed differential modulation of distinct metabolic pathways for C. difficile WT, luxS and B. fragilis upon co-culture, indicating that AI-2 may be involved in induction of selective metabolic responses in B. fragilis. Overall, our data suggest that C. difficile LuxS/AI-2 utilises different mechanisms to mediate formation of single and mixed species communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.