ArticleSaudi medical journal2019
The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additive. A novel in vitro study.
Article in Saudi medical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Do blood concentrates influence inflammatory signs and symptoms after mandibular third molar surgery? A systematic review and network meta-analysis of randomized clinical trials.Clinical oral investigations · 2023Pooled it
- Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies.Clinical oral investigations · 2020Pooled it
- Ameliorative role of silver nanoparticles incorporated with chitosan solution and leukocyte platelet-rich fibrin scaffold during colon anastomosis in rabbits.Journal of materials science. Materials in medicine · 2025Article
- How to explain the beneficial effects of leukocyte- and platelet-rich fibrin.Periodontology 2000 · 2025Review
- Efficacy of Blood Products and Photobiomodulation Therapy for Neurosensory Recovery in Patients With Inferior Alveolar Nerve Injury Following Orthognathic Surgery: A Systematic Review Study.Journal of lasers in medical sciences · 2025Review
- Review
- The Effects of Injectable Platelet-Rich Fibrin and Advanced-Platelet Rich Fibrin on Gingival Fibroblast Cell Vitality, Proliferation, Differentiation.Tissue engineering and regenerative medicine · 2023Article
- Article
- Standardization of Animal Models and Techniques for Platelet-Rich Fibrin Production: A Narrative Review and Guideline.Bioengineering (Basel, Switzerland) · 2023Review
- Can Our Blood Help Ensure Antimicrobial and Anti-Inflammatory Properties in Oral and Maxillofacial Surgery?International journal of molecular sciences · 2023Review
- Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells.International journal of molecular sciences · 2021Article
- Biocompatibility Evaluation of Human and Porcine Acellular Dermal Matrix on Human Primary Gingival Fibroblasts: In Vitro Comparative Study.European journal of dentistry · 2021Article
- Immediate implant placement in fresh extraction sockets.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2021Article
- The Effects of Leukocyte-Platelet Rich Fibrin (L-PRF) on Suppression of the Expressions of the Pro-Inflammatory Cytokines, and Proliferation of Schwann Cell, and Neurotrophic Factors.Scientific reports · 2020Article
- Flapless immediate implant placement into fresh molar extraction socket using platelet-rich fibrin: A case report.World journal of clinical cases · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo investigate the use of leukocyte-platelet rich fibrin on suppressing the porphyromonas gingivalis (PG-LPS)-induced secretion of proinflammatory cytokines. Methods:This quantitative experimental study was conducted at the School and Hospital of Stomatology, Jilin University, Changchun, China, between September 2017 and January 2019. A modified technique was used to obtain human gingival fibroblast cells (HGFCs). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Cell Counting Kit-8 tests were established to determine the proliferation rate. Human gingival fibroblast cells were treated by PG-LPS at different periods and the isolated mRNA was subjected to reverse transcription polymerase chain reaction and real time quantitative polymerase chain reaction. The release of platelet-derived growth factor and transforming-growth factor-β1 at various time intervals was observed. Results: We successfully established a modified technique for the production of HGFCs culture. One µg/mL PG-LPS was the recommended concentration to inhibit fibroblast proliferation. The expression of the pro-inflammatory cytokines messenger ribnucleic acid was notably raised at 3 and 6 hours post-PG-LPS treatment. The cumulative release of growth factors peaked during the first 24 hours and the production continued for 10 days. However, the fibroblast expression of cytokines was significantly suppressed after treatment with leucocyte- and platelet-rich fibrin (L-PRF). Conclusion: This study provided a novel way of obtaining HGFCs and greater understanding of the clinical impacts through the assessment of the anti-inflammatory properties of L-PRF in vitro.
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