Evidence map›Paper›PMID 31286947›Full record

ArticleBMC veterinary research2019

Genome sequence analysis reveals potential for virulence genes and multi-drug resistance in an Enterococcus faecalis 2A (XJ05) strain that causes lamb encephalitis.

Yingjin Chai, Xiaoxiao Gu, Qin Wu, Bingjiao Guo, Yayin Qi, Xiaolan Wang, Xia Zhou, Jie Li, Mengli Han, Fagang Zhong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Yingjin ChaiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China.
Xiaoxiao GuCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China.
Qin WuCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China.
Bingjiao GuoCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China.
Yayin QiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China.
Xiaolan WangCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China.
Xia ZhouCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China. GYYXiang@126.com.ORCID http://orcid.org/0000-0001-7461-5276
Jie LiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, China. hnmlss@163.com.
Mengli HanState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, 832000, Xinjiang, China. 605249596@qq.com.
Fagang ZhongState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, 832000, Xinjiang, China.
Shihezi University · CNXinjiang Academy of Agricultural and Reclamation Science · CN

Funding

National Natural Science Foundation of China 31260610National Natural Science Foundation of China (CN) 31160507The National Key Laboratory of Provincial and Ministerial co-construction for Sheep Genetic Improvement and Healthy Breeding Personnel Training Guidance Plan No.SKLGIH 2016A02
6 · The paper itself

Abstract

backgroundEnterococcus is an important component of normal flora in human and animals, but in recent years, the pathogenicity of Enterococcus has been confirmed in clinical medicine. More and more animal infections have been reported in veterinary clinics. For the last decades, outbreaks of encephalitis in lambs have become much more common in Northern Xinjiang, China. Consequent studies have confirmed that these affected lambs had been commonly infected with E. faecalis. More than 60 E. faecalis were isolated from the brain of infected lambs, A highly virulent strain entitled E. faecalis 2A (XJ05) were selected, sequenced and analyzed.

resultUsing whole genome sequence and de novo assembly, 18 contigs with NGS and annotation were obtained. It is confirmed that the genome has a size of 2.9 Mb containing 2783 protein-coding genes, as well as 54 tRNA genes and 4 rRNA genes. Some key features of this strain were identified, which included 7 predicted antibiotic resistance genes and 18 candidate virulence factor genes.

conclusionThe E. faecalis 2A (XJ05) genome is conspicuous smaller than E.faecalis V583, but not significantly different from other non-pathogenic E. faecalis. It carried 7 resistance genes including 4 kind of antibiotics which were consistent with the results of extensive drug resistance phenotypic, including aminoglycoside, macrolide, phenicol, and tetracycline. 2A (XJ05) also carried 18 new virulence factor genes related to virulence, hemolysin genes (cylA, cylB, cylM, cylL) may play an important role in lamb encephalitis by E. faecalis 2A (XJ05).

Indexed as

AnimalsDrug Resistance, BacterialDrug Resistance, MultipleEncephalitisEnterococcus faecalisGenome, BacterialSheepSheep DiseasesVirulenceE. faecalisLamb encephalitisWhole genome sequencing

Identifiers

PMID31286947
PMCPMC6615116
OpenAlexW2962241632

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.