Evidence map›Paper›PMID 31286881›Full record

ArticleBMC neuroscience2019

An enhanced staining method K-B-2R staining for three-dimensional nerve reconstruction.

Peng Luo, Jianghui Dong, Jian Qi, Yi Zhang, Xiaolin Liu, Yingchun Zhong, Cory J Xian, Liping Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Peng LuoDepartment of Bone and Joint Surgery, Shenzhen Sixth People's Hospital, Shenzhen, 518000, Guangdong, China.
Jianghui DongDepartment of Hand Surgery, Ningbo No. 6 Hospital, Ningbo, 315040, China.
Jian QiDepartment of Orthopedics Trauma and Microsurgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Yi ZhangDepartment of Plastic and Reconstructive Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Xiaolin LiuDepartment of Orthopedics Trauma and Microsurgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Yingchun ZhongSchool of Automation, Guangdong University of Technology, Guangzhou, 510006, China.
Cory J XianSchool of Pharmacy and Medical Sciences, and UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, 5001, Australia. cory.xian@unisa.edu.au.
Liping WangDepartment of Hand Surgery, Ningbo No. 6 Hospital, Ningbo, 315040, China. liping.wang@mymail.unisa.edu.au.ORCID 0000-0001-9355-1167
Sun Yat-sen University · CNUniversity of South Australia · AUGuangdong University of Technology · CNShenzhen Sixth People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThree-dimensional (3D) reconstruction of human peripheral nerves, as a useful tool to understand the nerve internal information and functional basis, has become an important area of research in the peripheral nerve field.

methodsIn this study, we proposed a two-dimensional (2D) Karnovsky-Roots toluidine blue ponceau 2R (K-B-2R) staining method based upon conventional Karnovsky-Roots staining. It significantly improved the ability to display nerve fascicles, motor and sensory nerve fiber textures. In this method, Karnovsky-Roots staining was carried out, followed by toluidine blue counterstain and ponceau 2R counterstain.

resultsComparisons were conducted between the three methods in staining of median nerve sections, which showed similar distribution characters in acetylcholinesterase-positive sites. The additional counterstaining did not change the basis of Karnovsky-Roots staining. However, the resulting images from this new method significantly facilitated the subsequent 3D nerve reconstruction and 3D printing.

conclusionsThese results show that the new staining method significantly enhanced the display qualities of nerve fascicle edges and fiber textures of motor and sensory nerves and facilitated 3D nerve reconstruction.

Indexed as

AcetylcholinesteraseAzo CompoundsHumansImaging, Three-DimensionalIndicators and ReagentsPeripheral NervesPrinting, Three-DimensionalStaining and LabelingThiocholineTolonium ChlorideAcetylcholinesteraseAzo CompoundsIndicators and Reagentsponceau MXThiocholineTolonium ChlorideAcetylcholinesterase stainingFascicle texture featureImage segmentationK-B-2RPeripheral nervesThree-dimensional reconstruction

Identifiers

PMID31286881
PMCPMC6615204
OpenAlexW2958845675

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.