ArticleBiomolecular detection and quantification2019
The kinetic requirements of extreme qPCR.
Article in Biomolecular detection and quantification, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Making Molecular Diagnostics Faster.International journal of laboratory hematology · 2026Review
- DYNAMIC: A Novel Software Implementation of a Kinetic Model of TaqMan PCR.Analytical chemistry · 2026Article
- Review
- ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay.Scientific reports · 2024Article
- Rapid Nucleic Acid Diagnostic Technology for Pandemic Diseases.Molecules (Basel, Switzerland) · 2024Review
- FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols.International journal of molecular sciences · 2024Article
- Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters.Diagnostics (Basel, Switzerland) · 2024Article
- Biological Applications of Thermoplasmonics.Nano letters · 2024Review
- Rational PCR Reactor Design in Microfluidics.Micromachines · 2023Review
- Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification.Micromachines · 2023Article
- Rapid Cycle and Extreme Polymerase Chain Reaction.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Ultra-rapid real-time microfluidic RT-PCR instrument for nucleic acid analysis.Lab on a chip · 2022Article
- Article
- A portable magnetofluidic platform for detecting sexually transmitted infections and antimicrobial susceptibility.Science translational medicine · 2021Article
- Nearest-neighbour transition-state analysis for nucleic acid kinetics.Nucleic acids research · 2021Article
- A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource Settings.Diagnostics (Basel, Switzerland) · 2020Article
- Appropriate Use and Future Directions of Molecular Diagnostic Testing.Current infectious disease reports · 2020Review
- Multiplexed Adaptive RT-PCR Based on L-DNA Hybridization Monitoring for the Detection of Zika, Dengue, and Chikungunya RNA.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The kinetic requirements of quantitative PCR were experimentally dissected into the stages of DNA denaturation, primer annealing, and polymerase extension. The temperature/time conditions for 2 stages were kept optimal, while the other was limited until the amplification efficiency decreased as measured by an increase in quantification cycle (Cq). Extension was studied in a commercial capillary LightCycler®. Using a rapid deletion mutant of Taq (KlenTaq
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.