ArticleScientific reports2019
Whole genome sequencing of amplified Plasmodium knowlesi DNA from unprocessed blood reveals genetic exchange events between Malaysian Peninsular and Borneo subpopulations.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review of asymptomatic Plasmodium knowlesi infection: an emerging challenge involving an emerging infectious disease.Malaria journal · 2022Pooled it
- Article
- Integrative genomics ofbioRxiv : the preprint server for biology · 2026Article
- Enhancing whole genome DNA amplification of Plasmodium falciparum for advanced molecular surveillance in malaria control.Malaria journal · 2025Article
- Article
- Genomic epidemiology of Plasmodium knowlesi reveals putative genetic drivers of adaptation in Malaysia.PLoS neglected tropical diseases · 2025Article
- Leveraging genomic insights from the neglected malaria parasites P. malariae and P. ovale using selective whole genome amplification (SWGA) approach.BMC genomics · 2025Article
- Emerging malaria in Indonesia: An overview ofParasite epidemiology and control · 2025Article
- Characterizing the genetic diversity and population structure of Plasmodium knowlesi in Aceh Province, Indonesia.PloS one · 2025Article
- Turning the needle into the haystack: Culture-independent amplification of complex microbial genomes directly from their native environment.PLoS pathogens · 2024Review
- Rapid profiling of Plasmodium parasites from genome sequences to assist malaria control.Genome medicine · 2023Article
- A fast machine-learning-guided primer design pipeline for selective whole genome amplification.PLoS computational biology · 2023Article
- Selective whole-genome amplification reveals population genetics of Leishmania braziliensis directly from patient skin biopsies.PLoS pathogens · 2023Article
- Population genetic analysis of Plasmodium knowlesi reveals differential selection and exchange events between Borneo and Peninsular sub-populations.Scientific reports · 2023Article
- Development and Optimization of a Selective Whole-Genome Amplification To Study Plasmodium ovale Spp.Microbiology spectrum · 2022Article
- Plasmodium knowlesi: the game changer for malaria eradication.Malaria journal · 2022Review
- Article
- A comparison of the clinical, laboratory and epidemiological features of two divergent subpopulations of Plasmodium knowlesi.Scientific reports · 2021Observational
- Characterizing the genomic variation and population dynamics of Plasmodium falciparum malaria parasites in and around Lake Victoria, Kenya.Scientific reports · 2021Article
- Malaria in the 'Omics Era'.Genes · 2021Review
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20 authors.
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Abstract
The zoonotic Plasmodium knowlesi parasite is the most common cause of human malaria in Malaysia. Genetic analysis has shown that the parasites are divided into three subpopulations according to their geographic origin (Peninsular or Borneo) and, in Borneo, their macaque host (Macaca fascicularis or M. nemestrina). Whilst evidence suggests that genetic exchange events have occurred between the two Borneo subpopulations, the picture is unclear in less studied Peninsular strains. One difficulty is that P. knowlesi infected individuals tend to present with low parasitaemia leading to samples with insufficient DNA for whole genome sequencing. Here, using a parasite selective whole genome amplification approach on unprocessed blood samples, we were able to analyse recent genomes sourced from both Peninsular Malaysia and Borneo. The analysis provides evidence that recombination events are present in the Peninsular Malaysia parasite subpopulation, which have acquired fragments of the M. nemestrina associated subpopulation genotype, including the DBPβ and NBPXa erythrocyte invasion genes. The NBPXb invasion gene has also been exchanged within the macaque host-associated subpopulations of Malaysian Borneo. Our work provides strong evidence that exchange events are far more ubiquitous than expected and should be taken into consideration when studying the highly complex P. knowlesi population structure.
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