ArticleScientific reports2019
BODIPY-cholesterol can be reliably used to monitor cholesterol efflux from capacitating mammalian spermatozoa.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- In Vitro Capacitation in Boar Sperm: Evaluation of Selected Detection Techniques.Methods and protocols · 2026Article
- Medium Composition Determines the Dynamics of Boar In Vitro Sperm Capacitation-Associated Events.International journal of molecular sciences · 2026Article
- SPINK3-Sperm Interaction Determines a Stable Sperm Subpopulation With Intact CatSper Channel.Journal of cellular physiology · 2026Article
- Fourier Transform Infrared Spectroscopy to Measure Cholesterol in Goat Spermatozoa.Animals : an open access journal from MDPI · 2025Article
- SPINK3-sperm interaction determines a stable sperm subpopulation with intact CatSper channel: SPINK3 stabilizes CatSper1 and membrane.bioRxiv : the preprint server for biology · 2025Article
- Using Cholesterol-Loaded Cyclodextrin to Improve Cryo-Survivability and Reduce Capacitation-Like Changes in Gender-Ablated Jersey Semen.Animals : an open access journal from MDPI · 2025Article
- Time to revise: impact of methodology on boar sperm capacitation in vitro via phosphotyrosine patterns.BMC veterinary research · 2025Review
- Effects of Cholesterol on the Breast Cancer Resistance Protein: Studies through the Synthesis and Biological Evaluation of Chemical Tools.ChemMedChem · 2025Article
- Boron-containing compounds as labels, drugs, and theranostic agents for diabetes and its complications.World journal of diabetes · 2024Article
- Characterization of acrosin and acrosin binding protein as novel CRISP2 interacting proteins in boar spermatozoa.Andrology · 2023Article
- Article
- Does Better Post-Thaw Motility of Dog Sperm Frozen with CLC Mean Better Zona Pellucida Binding Ability?Animals : an open access journal from MDPI · 2023Article
- Human sperm cooperate to transit highly viscous regions on the competitive pathway to fertilization.Communications biology · 2023Article
- The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†.Biology of reproduction · 2022Article
- Rhenium(I) conjugates as tools for tracking cholesterol in cells.Metallomics : integrated biometal science · 2022Article
- HDL mediates reverse cholesterol transport from ram spermatozoa and induces hyperactivated motility.Biology of reproduction · 2021Article
- Expression and Possible Role of Nicotinic Acetylcholine Receptor ε Subunit (AChRe) in Mouse Sperm.Biology · 2021Article
- Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation ImprovesFrontiers in cell and developmental biology · 2021Article
- Male Infertility: Shining a Light on Lipids and Lipid-Modulating Enzymes in the Male Germline.Journal of clinical medicine · 2020Review
- Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce CaFrontiers in physiology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capacitation is the final maturation step spermatozoa undergo prior to fertilisation. The efflux of cholesterol from the sperm membrane to the extracellular environment is a crucial step during capacitation but current methods to quantify this process are suboptimal. In this study, we validate the use of a BODIPY-cholesterol assay to quantify cholesterol efflux from spermatozoa during in vitro capacitation, using the boar as a model species. The novel flow cytometric BODIPY-cholesterol assay was validated with endogenous cholesterol loss as measured by mass spectrometry and compared to filipin labelling. Following exposure to a range of conditions, the BODIPY-cholesterol assay was able to detect and quantify cholesterol efflux akin to that measured with mass spectrometry. The ability to counterstain for viability is a unique feature of this assay that allowed us to highlight the importance of isolating viable cells only for a reliable measure of cholesterol efflux. Finally, the BODIPY-cholesterol assay proved to be the superior method to quantify cholesterol efflux relative to filipin labelling, though filipin remains useful for assessing cholesterol redistribution. Taken together, the BODIPY-cholesterol assay is a simple, inexpensive and reliable flow cytometric method for the measurement of cholesterol efflux from spermatozoa during in vitro capacitation.
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