Evidence map›Paper›PMID 31285389›Full record

ArticleBioscience reports2019

Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy.

Jiangwei Chen, Zunwen Lin, Kui Deng, Bin Shao, Dong Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jiangwei ChenDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Zunwen LinDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Kui DengDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Bin ShaoDepartment of Orthopedics, Jingdezhen First People's Hospital, Jingdezhen, China.
Dong YangDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, China dr_yangdong@126.com.ORCID 0000-0002-2322-0116
Nanchang University · CNJingdezhen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIntervertebral disc degeneration is a common degenerative disease. The present study aimed to explore the role and mechanism of tension-induced endoplasmic reticulum stress in intervertebral disc degeneration.

methodsIntervertebral disc degeneration models of SD rat were analyzed for apoptosis, the expression of Poly(ADP-ribose) polymerase (PARP), Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP using immunohistochemistry, qPCR and Western blot analysis. Annulus fibrosus cells of intervertebral disc were isolated, subjected to cyclic deformation stress and analyzed for ROS and apoptosis, lysosome activity and expression of genes. The cells were knockdown with siRNA or treated with endoplasmic reticulum stress inhibitor 4-PBA and assayed for ROS, apoptosis, lysosome activity and gene expression.

resultsCompared with the controls, intervertebral disc degeneration was observed through X-rays examinations and HS staining. Apoptosis and expression of PARP, Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP were significantly increased in the intervertebral disc tissue of the models. In mechanic mimic experiments, the primary annulus fibrosus cells were subjected to 18% cyclic deformation, ROS and apoptosis as well as the activity of lysosome were increased. Similarly, the expression of PARP, Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP was also increased significantly after deformation treatment. On other hand, when the cells were treated with 9 mM 4-PBA and/or CHOP-siRNA4, the apoptosis rate, ROS level, lysosome activity and expression of PARP, Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP were significantly reduced.

conclusionsAutophagy reaction mediated by endoplasmic reticulum stress plays important rale in tension-induced intervertebral disc degeneration. Intervertebral disc degeneration likely results from interactions between autophagy, apoptosis and reticulum stress, and is ROS-dependent.

Indexed as

AnimalsApoptosisAutophagyBeclin-1Caspase 12Caspase 3Endoplasmic Reticulum StressIntervertebral DiscIntervertebral Disc DegenerationLysosomesMalePoly(ADP-ribose) PolymerasesRatsRats, Sprague-DawleyRNA, Small InterferingTranscription Factor CHOPBeclin-1Caspase 12Caspase 3Poly(ADP-ribose) PolymerasesRNA, Small InterferingTranscription Factor CHOPautophagycyclic deformationendoplasmic reticulum stressintervertebral disc degeneration

Identifiers

PMID31285389
PMCPMC6684951
OpenAlexW2960475302

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.