ArticleMaterials (Basel, Switzerland)2019
3D Human Periodontal Stem Cells and Endothelial Cells Promote Bone Development in Bovine Pericardium-Based Tissue Biomaterial.
Article in Materials (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Orthodontic Extrusion in Daily Clinical Practice: Management of Fractured or Damaged Anterior Teeth.Journal of personalized medicine · 2025Review
- Geometrically modified bovine pericardium membrane promotes the expression of molecules targeted for a faster integration and vascularization process.Frontiers in bioengineering and biotechnology · 2024Article
- Mechanochemical coupling of MGF mediates periodontal regeneration.Bioengineering & translational medicine · 2024Article
- Flow-dependent shear stress affects the biological properties of pericyte-like cells isolated from human dental pulp.Stem cell research & therapy · 2023Article
- Review
- Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenvironment.Stem cells international · 2022Article
- Piezoelectric Signals in Vascularized Bone Regeneration.Biomolecules · 2021Review
- Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering.International journal of molecular sciences · 2021Article
- [Biocompatibility and effect on bone formation of a native acellular porcine pericardium: Results ofBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2021Article
- Review
- Angiogenic hydrogels for dental pulp revascularization.Acta biomaterialia · 2021Article
- Scaffold-Type Structure Dental Ceramics with Different Compositions Evaluated through Physicochemical Characteristics and Biosecurity Profiles.Materials (Basel, Switzerland) · 2021Article
- Functional Relationship between Osteogenesis and Angiogenesis in Tissue Regeneration.International journal of molecular sciences · 2020Review
- Bovine pericardium membrane, gingival stem cells, and ascorbic acid: a novel team in regenerative medicine.European journal of histochemistry : EJH · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone defects repair represents a public and urgent problem in clinical practice, in fact, every year, more than two million patients required new treatments for bone injuries. Today a complete vascularization is strategic in bone formation, representing a new frontier for clinical application. Aim of this research has been developed a three-dimensional (3D) coculture platform using a bovine pericardium collagen membrane (BioR) loaded with human periodontal ligament stem cells (hPDLSCs) and endothelial differentiated cells from hPDLSCs (E-hPDLSCs) able to undergo toward osteoangiogenesis differentiation process. First, we have characterized at confocal laser scanning microscopy (CLSM) level the E-hPDLSCs phenotype profile, through CD31 and CD34 markers expression and the ability to tube vessel formation. Real Time-Polimerase Chain Reaction (RT-PCR) and western blotting analyses revealed the upregulation of Runt-related transcription factor 2 (RUNX2), Collagen 1A1 (COL1A1), Vascular Endothelial Growth Factor-A (VEGF-A) genes and proteins in the living construct composed by hPDLSCs + E-hPDSCs/BioR. Human PDLSCs + E-hPDLSCs/BioR construct showed also an enhacement of de novo synthesis of osteocalcin. Given that, the extracellular-signal-regulated kinase (ERK)/mitogen activated protein kinase (MAPK) transduction signaling was involved in the osteogenesis and angiogenesis process, the ERK1/2 protein level at biochemical level, in our experimental model, has been investigated. Our results evidenced an upregulation of ERK1/2 proteins level born in the living construct. In conclusion, we believe that the use of the hPDLSCs and E-hPDLSCs coculture togheter with BioR as substrate, could represent an efficient model able to activate through ERK1/2 signaling pathway the osteoangiogenesis process, and then representing a new potential engineered platform for surgeons during the repair and the healing of bone defects.
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Registered trials
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