ArticlePloS one2019
Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Development of rapid and visual Erwinia amylovora detection technology using recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD).Pest management science · 2026Article
- Rapid On-Site Detection ofPlants (Basel, Switzerland) · 2026Article
- The evolution of next-generation lateral flow assays for bacterial and fungal diagnostics.Mikrochimica acta · 2026Review
- Establishment and clinical application of a rapid RPA-LFS assay for detectingFrontiers in cellular and infection microbiology · 2026Article
- Laboratory diagnosis of melioidosis.PLoS neglected tropical diseases · 2025Review
- Melioidosis molecular diagnostics: An update.Virulence · 2025Review
- Point-of-care detection of Burkholderia pseudomallei: A method integrating LAMP and LFSA with RNase HII hydrolysis.PLoS neglected tropical diseases · 2025Article
- An improved loop mediated isothermal amplification based assay for the rapid identification of genomic DNA ofCurrent research in microbial sciences · 2025Article
- Rapid and Accurate Detection ofInsects · 2024Article
- A Pan Plasmodium lateral flow recombinase polymerase amplification assay for monitoring malaria parasites in vectors and human populations.Scientific reports · 2024Article
- Development of a novel sequence based real-time PCR assay for specific and sensitive detection of Burkholderia pseudomallei in clinical and environmental matrices.Annals of clinical microbiology and antimicrobials · 2024Article
- Development of a Novel Internally Controlled HrpB1 Gene-Based Real-Time qPCR Assay for Detection of Burkholderia pseudomallei.Molecular diagnosis & therapy · 2024Article
- Propidium Monoazide (PMAxx)-Recombinase Polymerase Amplification Exo (RPA Exo) Assay for Rapid Detection ofMicroorganisms · 2023Article
- Single-tube one-step gel-based RT-RPA/PCR for highly sensitive molecular detection of HIV.The Analyst · 2023Article
- Specific biomarker mining and rapid detection ofFrontiers in microbiology · 2023Article
- Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a.PLoS neglected tropical diseases · 2022Article
- Specific Detection and Enumeration ofMicroorganisms · 2022Article
- A recombinase polymerase amplification lateral flow assay for rapid detection of Burkholderia pseudomallei, the causative agent of melioidosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Development of Multiplex PCR Assay for Screening of T6SS-5 Gene Cluster: TheDiagnostics (Basel, Switzerland) · 2022Article
- A New Rapid Method for the Authentication of Common Octopus (Foods (Basel, Switzerland) · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Melioidosis is a severe infectious disease caused by gram-negative, facultative intracellular pathogen Burkholderia pseudomallei (B. pseudomallei). Although cases are increasing reported from other parts of the world, it is an illness of tropical and subtropical climates primarily found in southeast Asia and northern Australia. Because of a 40% mortality rate, this life-threatening disease poses a public health risk in endemic area. Early detection of B. pseudomallei infection is vital for prognosis of a melioidosis patient. In this study, a novel isothermal recombinase polymerase amplification combined with lateral flow dipstick (LF-RPA) assay was established for rapid detection of B. pseudomallei. A set of primer-probe targeting orf2 gene within the putative type III secretion system (T3SS) cluster genes was generated and parameters for the LF-RPA assay were optimized. Result can be easy visualized in 30 minutes with the limit of detection (LOD) as low as 20 femtogram (fg) (ca. 25.6 copies) of B. pseudomallei genomic DNA without a specific equipment. The assay is highly specific as no cross amplification was observed with Burkholderia mallei, members of the Burkholderia cepacia-complex and 35 non-B. pseudomallei bacteria species. Moreover, isolates from patients in Hainan (N = 19), Guangdong (N = 1), Guangxi (N = 3) province of China as well as in Australia (N = 3) and Thailand (N = 1) were retrospectively confirmed by the newly developed method. LODs for B. pseudomallei-spiked soil and blood samples were 2.1×103 CFU/g and 4.2×103 CFU/ml respectively. The sensitivity of the LF-RPA assay was comparable to TaqMan Real-Time PCR (TaqMan PCR). In addition, the LF-RPA assay exhibited a better tolerance to inhibitors in blood than TaqMan PCR. Our results showed that the LF-RPA assay is an alternative to existing PCR-based methods for detection of B. pseudomallei with a potentiality of early accurate diagnosis of melioidosis at point of care or in-field use.
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