ArticlePLoS neglected tropical diseases2019
Development of multiplex PCR for neglected infectious diseases.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- Multiplex PCR-Lateral Flow Dipstick Method for Detection of Thermostable Direct Hemolysin (Biosensors · 2023Article
- Development of Multiplex PCR Assay for Screening of T6SS-5 Gene Cluster: TheDiagnostics (Basel, Switzerland) · 2022Article
- Ending the Neglect of Treatable Bacterial Zoonoses Responsible for Non-Malaria Fevers.The Yale journal of biology and medicine · 2021Review
- Current progress on COVID-19 related to biosensing technologies: New opportunity for detection and monitoring of viruses.Microchemical journal : devoted to the application of microtechniques in all branches of science · 2021Review
- Turfgrass Disease Diagnosis: Past, Present, and Future.Plants (Basel, Switzerland) · 2020Review
- A Microfluidic Diagnostic Device Capable of Autonomous Sample Mixing and Dispensing for the Simultaneous Genetic Detection of Multiple Plant Viruses.Micromachines · 2020Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scrub typhus, murine typhus, and leptospirosis are widely neglected infectious diseases caused by Orientia tsutsugamushi, Rickettsia typhi, and pathogenic Leptospira spp., respectively. Patients usually present with non-specific symptoms and therefore are commonly diagnosed with acute undifferentiated febrile illness. Consequently, patients face delayed treatment and increased mortality. Antibody-based serological test currently used as gold standard has limitations due to insufficient antibody titers, especially in the early phase of infection. In this study, we aimed to develop multiplex PCR to combine 3 primer pairs that target specific genes encoding 56-kDa TSA of O. tsutsugamushi, 17-kDa antigen of R. typhi, and LipL32 of L. Interrogans and evaluate its performance in comparison to the standard serological tests. Using EDTA blood samples of known patients, the sensitivity and specificity of our multiplex PCR was 100% and 70%, respectively. In addition, the assay was able to diagnose the co-infection of scrub typhus and leptospirosis. The assay may be useful in identifying causative agents during the early phase of these diseases, enabling prompt and appropriate treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.