Evidence map›Paper›PMID 31283017›Full record

ArticleAlcoholism, clinical and experimental research2019

Different Effects of Knockouts in ALDH2 and ACSS2 on Embryonic Stem Cell Differentiation.

Ryan N Serio, Changyuan Lu, Steven S Gross, Lorraine J Gudas

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Alcoholism, clinical and experimental research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. cGAS Inhibits ALDH2 to Suppress Lipid Droplet Function and Regulate MASLD Progression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Ryan N SerioWeill Cornell Graduate School of Medical Sciences of Cornell University, New York, New York.
Changyuan LuDepartment of Pharmacology, Weill Cornell Medical College, New York, New York.
Steven S GrossWeill Cornell Graduate School of Medical Sciences of Cornell University, New York, New York.
Lorraine J GudasWeill Cornell Graduate School of Medical Sciences of Cornell University, New York, New York.ORCID 0000-0003-3115-4777
Cornell University · US

Funding

CANCER PHARMACOLOGYT32CA062948 · NCI · WEILL MEDICAL COLL OF CORNELL UNIV · PI LORRAINE J GUDAS · 1994 to 2026
$12.1M
Diet and Alcohol Induced Epigenetic Changes in Oral Cavity Carcinogenesis ModelR01AA018332 · NIAAA · WEILL MEDICAL COLL OF CORNELL UNIV · PI GUDAS, LORRAINE J · 2010 to 2014
$2.1M
Alcohol-Induced Epigenetic Changes in Stem CellsR21AA021484 · NIAAA · WEILL MEDICAL COLL OF CORNELL UNIV · PI GUDAS, LORRAINE J · 2012 to 2013
$430k
Ethanol Metabolism in Embryonic Stem CellsF31AA024024 · NIAAA · WEILL MEDICAL COLL OF CORNELL UNIV · PI SERIO, RYAN N · 2015 to 2018
$132k
NCI NIH HHS T32 CA062948NIAAA NIH HHS F31 AA024024NIAAA NIH HHS R01 AA018332NIAAA NIH HHS R21 AA021484
6 · The paper itself

Abstract

backgroundEthanol (EtOH) is a teratogen that causes severe birth defects, but the mechanisms by which EtOH affects stem cell differentiation are unclear. Our goal here is to examine the effects of EtOH and its metabolites, acetaldehyde (AcH) and acetate, on embryonic stem cell (ESC) differentiation.

methodsWe designed ESC lines in which aldehyde dehydrogenase (ALDH2, NCBI#11669) and acyl-CoA synthetase short-chain family member 2 (ACSS2, NCBI#60525) were knocked out by CRISPR-Cas9 technology. We selected these genes because of their key roles in EtOH oxidation in order to dissect the effects of EtOH metabolism on differentiation.

resultsBy using kinetic assays, we confirmed that AcH is primarily oxidized by ALDH2 rather than ALDH1A2. We found increases in mRNAs of differentiation-associated genes (Hoxa1, Cyp26a1, and RARβ2) upon EtOH treatment of WT and Acss2

conclusionsACSS2 knockouts showed no changes in differentiation phenotype, while pluripotency-related transcripts were decreased in ALDH2 knockout ESCs. We demonstrate that AcH increases differentiation-associated mRNAs in ESCs via RARγ.

Indexed as

AcetaldehydeAcetate-CoA LigaseAldehyde Dehydrogenase, MitochondrialAnimalsCell DifferentiationEmbryonic Stem CellsEthanolGene Knockout TechniquesKruppel-Like Factor 4MiceReceptors, Retinoic AcidRetinoic Acid Receptor gammaAcetaldehydeAcetate-CoA LigaseACSS2 protein, mouseAldehyde Dehydrogenase, MitochondrialALDH2 protein, mouseEthanolKlf4 protein, mouseKruppel-Like Factor 4Receptors, Retinoic AcidRetinoic Acid Receptor gamma4-HNEAcetaldehydeACSS2AlcoholALDH2DifferentiationEthanolRARγRetinoic AcidStem Cells

Identifiers

PMID31283017
PMCPMC6722009
OpenAlexW2958695611

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.