ArticleJournal of molecular cell biology2020
SP1 governs primordial folliculogenesis by regulating pregranulosa cell development in mice.
Article in Journal of molecular cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Beyond apoptosis: evidence of other regulated cell death pathways in the ovary throughout development and life.Human reproduction update · 2023Pooled it
- Spatiotemporally primed coelomic epithelium and supporting-like cells establish the dual origin of granulosa cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- DNA methylation profiling of the granulosa layer reveals epigenetic features of excessive hierarchical follicle development in chickens.Poultry science · 2026Article
- Species-specific roles of SP1 in bovine and human embryonic genome activation and early embryonic development.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- HDAC3 Preserves the primordial follicle reserve by epigenetically suppressing ferroptosis in pregranulosa cells.Cellular and molecular life sciences : CMLS · 2026Article
- Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Ovarian development is driven by early spatiotemporal priming of the coelomic epithelium.bioRxiv : the preprint server for biology · 2025Article
- Single-Cell Transcriptomic Analysis of the Potential Mechanisms of Follicular Development inInternational journal of molecular sciences · 2025Article
- Characterizing the Ovarian Cytogenetic Dynamics of Sichuan Bream (International journal of molecular sciences · 2025Article
- Mechanisms of follicular atresia: focus on apoptosis, autophagy, and ferroptosis.Frontiers in endocrinology · 2025Review
- Protective effect of luteinizing hormone on frozen-thawed ovarian follicles and granulosa cells.PloS one · 2025Article
- ELK1 regulates BMPR1B transcriptional activity in ovine granulosa cells.Frontiers in cell and developmental biology · 2025Article
- Premeiotic deletion of Eif2s2 causes oocyte arrest at the early diplotene stage and apoptosis in mice.Cell proliferation · 2024Article
- EZH1/2 plays critical roles in oocyte meiosis prophase I in mice.Biological research · 2024Article
- RRM2 promotes the proliferation of chicken myoblasts, inhibits their differentiation and muscle regeneration.Poultry science · 2024Article
- Analysis ofGenes · 2023Article
- Single-cell RNA-seq identified novel genes involved in primordial follicle formation.Frontiers in endocrinology · 2023Article
- Application of Single-Cell RNA Sequencing in Ovarian Development.Biomolecules · 2022Review
- ASH1L contributes to oocyte apoptosis by regulating DNA damage.American journal of physiology. Cell physiology · 2022Article
- Oocyte Casein kinase 1α deletion causes defects in primordial follicle formation and oocyte loss by impairing oocyte meiosis and enhancing autophagy in developing mouse ovary.Cell death discovery · 2022Article
Corrections and comments
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Authors and funding
18 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Establishment of the primordial follicle (PF) pool is pivotal for the female reproductive lifespan; however, the mechanism of primordial folliculogenesis is poorly understood. Here, the transcription factor SP1 was shown to be essential for PF formation in mice. Our results showed that SP1 is present in both oocytes and somatic cells during PF formation in the ovary. Knockdown of Sp1 expression, especially in pregranulosa cells, significantly suppressed nest breakdown, oocyte apoptosis, and PF formation, suggesting that SP1 expressed by somatic cells functions in the process of primordial folliculogenesis. We further demonstrated that SP1 governs the recruitment and maintenance of Forkhead box L2-positive (FOXL2+) pregranulosa cells using an Lgr5-EGFP-IRES-CreERT2 (Lgr5-KI) reporter mouse model and a FOXL2+ cell-specific knockdown model. At the molecular level, SP1 functioned mainly through manipulation of NOTCH2 expression by binding directly to the promoter of the Notch2 gene. Finally, consistent with the critical role of granulosa cells in follicle survival in vitro, massive loss of oocytes in Sp1 knockdown ovaries was evidenced before puberty after the ovaries were transplanted under the renal capsules. Conclusively, our results reveal that SP1 controls the establishment of the ovarian reserve by regulating pregranulosa cell development in the mammalian ovary.
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