ArticleAdipocyte2019
Influence of glucose and insulin in human adipogenic differentiation models with adipose-derived stem cells.
Article in Adipocyte, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Optimized Metabolic Conditions Reveal the Adipogenic Competence of Wharton's Jelly MSCs: Insight from Literature Review and Experimental Validation.Stem cell reviews and reports · 2026Review
- Enzymatically triggered glucose-releasing scaffolds as nutritional supports for millimeter-scale tissue engineering.Materials today. Bio · 2026Article
- Mitochondrial Abundant Heat Soluble (MAHS) Protein Expression Modulates Metabolic Dynamics in Human Adipose-Derived Stem Cells.International journal of molecular sciences · 2026Article
- E3 ligase FBXO2-mediated protein stability of insulin receptor regulates adipogenesis and metabolic health in obesity.Cell death & disease · 2026Article
- Seeding of Dermal Substitutes with Glucose-Pretreated Nanofat Accelerates In Vivo Vascularization and Tissue Integration.Journal of functional biomaterials · 2025Article
- Development of optimized differentiation media for murine bone marrow multipotent mesenchymal stromal cells.Histochemistry and cell biology · 2025Article
- The role of lncRNA in the differentiation of adipose-derived stem cells: from functions to mechanism.Journal of molecular medicine (Berlin, Germany) · 2025Review
- Inclusion of calcium propionate in late gestation protein supplements increases subsequent offspring marbling scores in range beef cows.Translational animal science · 2025Article
- Functional impacts of lactylation in Hypoxia‒primed mesenchymal stromal cells.Frontiers in cell and developmental biology · 2025Review
- Fibro-adipogenic progenitors in physiological adipogenesis and intermuscular adipose tissue remodeling.Molecular aspects of medicine · 2024Review
- Interaction between peripheral blood mononuclear cells andFrontiers in immunology · 2024Article
- An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis.Adipocyte · 2023Article
- D-mannose reduces adipogenesis by inhibiting the PI3K/AKT signaling pathway.Histology and histopathology · 2023Article
- Review
- Article
- Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells.Stem cell research & therapy · 2022Article
- Adipose tissue-derived stem cells: a comparative review on isolation, culture, and differentiation methods.Cell and tissue banking · 2022Review
- High-throughput drug screening models of mature adipose tissues which replicate the physiology of patients' Body Mass Index (BMI).Bioactive materials · 2022Article
- Article
- Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular Matrices.Molecules (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autologous fat grafting represents an attractive source for tissue engineering applications in the field of reconstructive medicine. However, in adipogenic differentiation protocols for human adipose-derived stem cells, the concentration of glucose and insulin varies considerably. With the intent to gain maximum tissue augmentation, we focused on the late phase of adipogenesis. In this study, we modified the differentiation protocol for adipose-derived stem cells by prolongation of the induction period and the application highly concentrated glucose and insulin. Human adipose-derived stem cells were isolated from subcutaneous depots and differentiated in a standard induction medium for the first two weeks, followed by two weeks with varying glucose and insulin concentrations. Morphological changes assessed using Oil-Red-O staining were examined for corresponding alterations in the expression of the adipogenic markers peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase (LPL). Furthermore, glucose and lactate levels in conditioned media were monitored over the period of differentiation. We found high-glucose media increasing the level of lipid accumulation and the size of single droplets whereas insulin significantly showed a dose-dependent negative effect on fat storage. However, whereas high glucose stimulated PPARγ transcription, expression levels in insulin-treated cells remained constant. Results permit assumptions that a high-glucose medium intensifies the degree of differentiation in mature adipocytes providing conditions to promote graft volume while we have identified highly concentrated insulin treatment as an inhibitor of lipid storage in the late adipogenic differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.