Evidence map›Paper›PMID 31279833›Full record

ArticleProtein expression and purification2019

Overproduction and purification of highly active recombinant Pseudomonas aeruginosa str. PAO1 RNA polymerase holoenzyme complex.

Derrick Afful, Liming Cai, Cory Momany

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Derrick AffulDepartment of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, 30602, USA.
Liming CaiDepartment of Computer Sciences, University of Georgia, Athens, GA, 30602, USA.
Cory MomanyDepartment of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, 30602, USA. Electronic address: cmomany@uga.edu.
University of Georgia · US

Funding

From RNA Sequence to 3D structure, Accurate Prediction Through Backbone K-TreesR01GM117596 · NIGMS · UNIVERSITY OF GEORGIA · PI CAI, LIMING · 2015 to 2017
$641k
NIGMS NIH HHS R01 GM117596
6 · The paper itself

Abstract

The bacterial RNA polymerase (RNAP) is a large, complex molecular machine that is the engine of gene expression. Despite global conservation in their structures and function, RNAPs from different bacteria can have unique features in promoter and transcription factor recognition. Therefore, availability of purified RNAP from different bacteria is key to understanding these species-specific aspects and will be valuable for antibiotic drug discovery. Pseudomonas aeruginosa is one of the leading causes of hospital and community acquired infections worldwide - making the organism an important public health pathogen. We developed a method for producing high quantities of highly pure and active recombinant P. aeruginosa str. PAO1 RNAP core and holoenzyme complexes that employed two-vector systems for expressing the core enzyme (α, β, β', and ω subunits) and for expressing the holoenzyme complex (core + σ

Indexed as

Bacterial ProteinsCloning, MolecularDNA-Directed RNA PolymerasesEscherichia coliHoloenzymesMultienzyme ComplexesPseudomonas aeruginosaRecombinant ProteinsBacterial ProteinsDNA-Directed RNA PolymerasesHoloenzymesMultienzyme ComplexesRecombinant ProteinsAnion exchangeCo-expression of protein subunitsEscherichia coliMultisubunit-complexNucleic acid bindingNucleic acid metabolismPseudomonas aeruginosaRNA polymeraseTranscription

Identifiers

PMID31279833
PMCPMC6688924
OpenAlexW2956061982

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.