ArticleMicrobiome2019
Microbial differences between dental plaque and historic dental calculus are related to oral biofilm maturation stage.
Article in Microbiome, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers, 1 of them a synthesis that pooled it.
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Who cites it
88 citing papers in PubMed, 1 synthesis or guideline pooled it, 170 citations in OpenAlex.
- Poor glycemic control impairs oral health in children with type 1 diabetes mellitus - a systematic review and meta-analysis.BMC oral health · 2024Pooled it
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- The Oral Microbiome of King Richard III of England.American journal of biological anthropology · 2026Article
- Ancient Species Diversity and Niche Adaptation in Tannerella and Porphyromonas Revealed Through Pangenomics.Genome biology and evolution · 2026Article
- Tobacco-Induced Oral Dysbiosis and Microbial Shifts: A Narrative Review of Their Role in Systemic Inflammation and Disease.Microorganisms · 2026Review
- In vitro evaluation of dentin surface alterations induced by a helical ultrasonic scaler tip using coherence correlation interferometry.Clinical oral investigations · 2026Article
- The genomic history of Streptococcus mutans from the Mesolithic until modern times.Genome biology · 2026Article
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- Effect of antimicrobial oral rinse use on prognostic scores in critically ill patients.Brazilian oral research · 2026Article
- Innate Immunity Gene Variants Affect Periodontitis Susceptibility.Advances in experimental medicine and biology · 2026Article
- Host Traits Impact the Outcome of Metagenomic Library Preparation From Dental Calculus Samples Across Diverse Mammals.Molecular ecology resources · 2025Article
- Ancient DNA and biomarkers from artefacts: insights into technology and cultural practices in Neolithic Europe.Proceedings. Biological sciences · 2025Article
- SANA-Biome: A Protocol for a Cross-Sectional Study on Oral Health, Diet, and the Oral Microbiome in Romania.Healthcare (Basel, Switzerland) · 2025Article
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- Periodontal Microbial Profiles Across Periodontal Conditions in Pediatric Subjects: A Narrative Review.Microorganisms · 2025Review
- Considerations in the treatment of individuals with obesity and periodontitis.Clinical obesity · 2025Review
- The impact of dietary interventions on cardiometabolic health.Cardiovascular diabetology · 2025Review
- Biofilm Resilience: Molecular Mechanisms Driving Antibiotic Resistance in Clinical Contexts.Biology · 2025Review
- Streptococcus abundance and oral site tropism in humans and non-human primates reflects host and lifestyle differences.NPJ biofilms and microbiomes · 2025Article
- Meta-omics reveals subgingival plaque reconstruction dynamics.Journal of oral microbiology · 2025Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDental calculus, calcified oral plaque biofilm, contains microbial and host biomolecules that can be used to study historic microbiome communities and host responses. Dental calculus does not typically accumulate as much today as historically, and clinical oral microbiome research studies focus primarily on living dental plaque biofilm. However, plaque and calculus reflect different conditions of the oral biofilm, and the differences in microbial characteristics between the sample types have not yet been systematically explored. Here, we compare the microbial profiles of modern dental plaque, modern dental calculus, and historic dental calculus to establish expected differences between these substrates.
resultsMetagenomic data was generated from modern and historic calculus samples, and dental plaque metagenomic data was downloaded from the Human Microbiome Project. Microbial composition and functional profile were assessed. Metaproteomic data was obtained from a subset of historic calculus samples. Comparisons between microbial, protein, and metabolomic profiles revealed distinct taxonomic and metabolic functional profiles between plaque, modern calculus, and historic calculus, but not between calculus collected from healthy teeth and periodontal disease-affected teeth. Species co-exclusion was related to biofilm environment. Proteomic profiling revealed that healthy tooth samples contain low levels of bacterial virulence proteins and a robust innate immune response. Correlations between proteomic and metabolomic profiles suggest co-preservation of bacterial lipid membranes and membrane-associated proteins.
conclusionsOverall, we find that there are systematic microbial differences between plaque and calculus related to biofilm physiology, and recognizing these differences is important for accurate data interpretation in studies comparing dental plaque and calculus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.