ArticleScientific reports2019
Enhancement of laccase activity by pre-incubation with organic solvents.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
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- Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds.Biotechnology letters · 2024Article
- Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization.PloS one · 2024Article
- Role of microbial laccases in valorization of lignocellulosic biomass to bioethanol.Frontiers in bioengineering and biotechnology · 2024Review
- Recent Prospects of Carbonaceous Nanomaterials-Based Laccase Biosensor for Electrochemical Detection of Phenolic Compounds.Biosensors · 2023Review
- A comparative screening of laccase-mediator systems by white-rot fungi laccases for biocatalytic benzyl alcohol oxidation.Scientific reports · 2022Article
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- Identification and Characterization of a NewFrontiers in microbiology · 2022Article
- An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green.International journal of molecular sciences · 2021Article
- Laccase-Catalyzed 1,4-Dioxane-Mediated Synthesis of BelladineInternational journal of molecular sciences · 2021Article
- London Dispersion in Alkane Solvents.Angewandte Chemie (International ed. in English) · 2021Article
- Production of Recombinant Laccase FromFrontiers in bioengineering and biotechnology · 2021Article
- Novel Thermophilic Bacterial Laccase for the Degradation of Aromatic Organic Pollutants.Frontiers in chemistry · 2021Article
- Optimisation of the Production and Bleaching Process for a New Laccase from Madurella mycetomatis, Expressed in Pichia pastoris: from Secretion to Yielding Prominent.Molecular biotechnology · 2021Article
- The crucial role of bacterial laccases in the bioremediation of petroleum hydrocarbons.World journal of microbiology & biotechnology · 2020Review
- Applicability of Recombinant Laccases From the White-Rot FungusFrontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laccases that are tolerant to organic solvents are powerful bio-catalysts with broad applications in biotechnology. Most of these uses must be accomplished at high concentration of organic solvents, during which proteins undergo unfolding, thereby losing enzyme activity. Here we show that organic-solvent pre-incubation provides effective and reversible 1.5- to 4.0-fold enhancement of enzyme activity of fungal laccases. Several organic solvents, including acetone, methanol, ethanol, DMSO, and DMF had an enhancement effect among all laccases studied. The enhancement was not substrate-specific and could be observed by using both phenolic and non-phenolic substrates. Laccase preincubated with organic solvents was sensitive to high temperature but remained stable at 25 °C, for an advantage for long-term storage. The acetone-pre-incubated 3-D structure of DLac, a high-efficiency fungal laccase, was determined and confirmed that the DLac protein structure remains intact and stable at a high concentration of organic solvent. Moreover, the turnover rates of fungal laccases were improved after organic-solvent pre-incubation, with DLac showing the highest enhancement among the fungal laccases examined. Our investigation sheds light on improving fungal laccase usage under extreme conditions and extends opportunities for bioremediation, decolorization, and organic synthesis.
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Registered trials
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