ArticleScientific reports2019
Selection of reference genes for flowering pathway analysis in the masting plants, Celmisia lyallii and Chionochloa pallens, under variable environmental conditions.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Cross-species stability of reference genes in medicinal plants Arnica montana and Arnica chamissonis.Scientific reports · 2024Article
- Evaluating and Validating Sunflower Reference Genes for Q-PCR Studies Under High Temperature Condition.Iranian journal of biotechnology · 2023Article
- Natural disturbances and masting: from mechanisms to fitness consequences.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Identification and Characterization of Perennial Ryegrass (Frontiers in plant science · 2021Article
- Optimal reference genes for gene expression analysis in polyploid of Cyprinus carpio and Carassius auratus.BMC genetics · 2020Article
- Selection and validation of reliable reference genes for gene expression studies from Monilinia vaccinii-corymbosi infected wild blueberry phenotypes.Scientific reports · 2020Article
- Visceral leishmaniasis: a practical strategy for quantitative molecular diagnosis in naturally infected dogs.Parasitology research · 2020Article
- Article
- Identification of flowering-time genes in mast flowering plants using De Novo transcriptomic analysis.PloS one · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mast flowering is characterised by mass synchronised flowering at irregular intervals over a wide geographical area. An understanding of the molecular drivers of mast flowering requires expression analysis of key developmentally regulated gene(s). Reverse transcription-quantitative PCR is the gold standard technique used to assess expression of target gene(s) and to validate high-throughput sequencing data. Selection and validation of appropriate reference gene(s), used as normalisation factors in transcript abundance analysis, is an essential step to avoid ambiguous expression results. Eight candidate reference genes were assessed to select the best internal normalisation factors in naturally growing masting plants Chionochloa pallens and Celmisia lyallii. Statistical packages geNorm, Normfinder, BestKeeper, ΔC
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