ArticlePloS one2019
Assessment of malaria real-time PCR methods and application with focus on low-level parasitaemia.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Misidentification of Plasmodium ovale as Plasmodium vivax malaria by a microscopic method: a meta-analysis of confirmed P. ovale cases.Scientific reports · 2020Pooled it
- Current practices and innovative diagnostics for Malaria in Ethiopia: a scoping review.BMC infectious diseases · 2026Article
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- α-Thalassemia has no association with asymptomatic Plasmodium falciparum carriage in three ecological zones of Ghana.Scientific reports · 2026Article
- Improving diagnostics and surveillance of malaria among displaced people in Africa.International journal for equity in health · 2025Review
- Article
- Challenges in Diagnosing and Treating Acutely Febrile Children with Suspected Malaria at Health Care Facilities in the Lake Mwanza Region of Tanzania.The American journal of tropical medicine and hygiene · 2024Article
- The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non-Endemic Areas.International journal of molecular sciences · 2024Review
- Detection of novelFrontiers in cellular and infection microbiology · 2024Article
- PET-PCR reveals low parasitaemia and submicroscopic malarial infections in Honduran Moskitia.Malaria journal · 2023Article
- Antimalarial Drug Resistance: A Brief History of Its Spread in Indonesia.Drug design, development and therapy · 2023Review
- A Rapid FRET Real-Time PCR Protocol for Simultaneous Quantitative Detection and Discrimination of HumanBio-protocol · 2022Article
- Malaria prevalence and performance of diagnostic tests among patients hospitalized with acute undifferentiated fever in Zanzibar.Malaria journal · 2022Article
- Nationwide molecular surveillance of three Plasmodium species harboured by symptomatic malaria patients living in Ghana.Parasites & vectors · 2022Article
- Evaluating performance of multiplex real time PCR for the diagnosis of malaria at elimination targeted low transmission settings of Ethiopia.Malaria journal · 2022Article
- Potential Opportunities and Challenges of Deploying Next Generation Sequencing and CRISPR-Cas Systems to Support Diagnostics and Surveillance Towards Malaria Control and Elimination in Africa.Frontiers in cellular and infection microbiology · 2022Review
- Advances and challenges in automated malaria diagnosis using digital microscopy imaging with artificial intelligence tools: A review.Frontiers in microbiology · 2022Review
- Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia.Malaria journal · 2021Article
- An Optimized Real-Time qPCR Method for the Effective Detection of Human Malaria Infections.Diagnostics (Basel, Switzerland) · 2021Article
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In epidemiological surveys and surveillance the application of molecular tools is essential in detecting submicroscopic malaria. A genus-specific conventional cytochrome b (cytb) PCR has shown high sensitivity in field studies, detecting 70% submicroscopic malaria. The main objective of this study was to assess the conversion from conventional to real-time PCR testing both SYBR and probe protocols, and including quantitative (q) PCR. The protocols were assessed applying well-defined clinical patient material consisting of 33 positive and 80 negative samples. Sequencing of positive PCR products was performed. In addition, a sensitivity comparison of real-time PCR methods was done by including five relevant assays investigating the effect of amplification target and platform. Sensitivity was further examined using field material consisting of 111 P.falciparum positive samples from Tanzanian children (< 5 years), as well as using related patient data to assess the application of q-PCR with focus on low-level parasitaemia. Both the cytb SYBR and probe PCR protocols showed as high sensitivity and specificity as their conventional counterpart, except missing one P. malariae sample. The SYBR protocol was more sensitive and specific than using probe. Overall, choice of amplification target applied is relevant for achieving ultra-sensitivity, and using intercalating fluorescence dye rather than labelled hydrolysis probes is favourable. Application of q-PCR analysis in field projects is important for the awareness and understanding of low-level parasitaemia. For use in clinical diagnosis and epidemiological studies the highly sensitive and user-friendly cytb SYBR q-PCR method is a relevant tool. The genus-specific method has the advantage that species identification by sequencing can be performed as an alternative to species-specific PCR.
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