ArticleThe Journal of experimental medicine1988
Point mutations cause the somatic diversification of IgM and IgG2a antiphosphorylcholine antibodies.
Article in The Journal of experimental medicine, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 64 citations in OpenAlex.
- Somatic immunoglobulin hypermutation.Current opinion in immunology · 2002Review
- A role for RAD51 in the generation of immunoglobulin gene diversity in rabbits.Journal of immunology (Baltimore, Md. : 1950) · 1999Article
- The transcriptional promoter regulates hypermutation of the antibody heavy chain locus.The Journal of experimental medicine · 1997Article
- Enhancement and destruction of antibody function by somatic mutation: unequal occurrence is controlled by V gene combinatorial associations.The EMBO journal · 1995Article
- Defective secretion of an immunoglobulin caused by mutations in the heavy chain complementarity determining region 2.The Journal of experimental medicine · 1994Article
- Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.The Journal of experimental medicine · 1994Article
- Isolation of germinal centerlike events from human spleen RNA. Somatic hypermutation of a clonally related VH6DJH rearrangement expressed with IgM, IgG, and IgA.The Journal of clinical investigation · 1993Article
- Somatic hypermutation of an immunoglobulin mu heavy chain transgene.The Journal of experimental medicine · 1993Article
- Germ line variable regions that match hypermutated sequences in genes encoding murine anti-hapten antibodies.Genetics · 1992Article
- Analysis of a T cell receptor gene as a target of the somatic hypermutation mechanism.The Journal of experimental medicine · 1992Article
- Immunoglobulin heavy chain gene expression in peripheral blood B lymphocytes.The Journal of clinical investigation · 1992Article
- Mutation pattern of immunoglobulin transgenes is compatible with a model of somatic hypermutation in which targeting of the mutator is linked to the direction of DNA replication.The EMBO journal · 1991Article
- Somatic mutation in constant regions of mouse lambda 1 light chains.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Altered reactivity of immunoglobulin produced by human-human hybridoma cells transfected by pSV2-neo gene.Cytotechnology · 1991Article
- Boundaries of somatic mutation in rearranged immunoglobulin genes: 5' boundary is near the promoter, and 3' boundary is approximately 1 kb from V(D)J gene.The Journal of experimental medicine · 1990Article
- Heavy-chain class switch does not terminate somatic mutation.The Journal of experimental medicine · 1990Article
- Parallel evolution of antibody variable regions by somatic processes: consecutive shared somatic alterations in VH genes expressed by independently generated hybridomas apparently acquired by point mutation and selection rather than by gene conversion.The Journal of experimental medicine · 1990Article
- Evolution of antibody structure during the immune response. The differentiative potential of a single B lymphocyte.The Journal of experimental medicine · 1989Article
- Protein evolution on rugged landscapes.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Significant structural and functional change of an antigen-binding site by a distant amino acid substitution: proposal of a structural mechanism.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The genetic mechanism responsible for the somatic diversification of two mAbs was determined. The two PC-binding hybridomas were representative of events early and late in the immune response. The P28 cell line that produces an IgM antibody and thus represents events early in the immune response, was found to have 3 bp changes in its heavy chain variable (VH) region, with some changes in antibody affinity or specificity. The RP93 cell line that produces an IgG2a antibody and thus represents later events in the immune response, was found to have 9 bp changes in its VH region resulting in decreased affinity for PC and altered specificity. Oligonucleotides specific for linked base changes in the second hypervariable regions of both of these antibodies were used to look for previously undescribed V regions or other donor sequences that could have been responsible for these base changes. Since no donor sequences were found, we have concluded that somatic point mutation rather than gene conversion, V region replacement or the expression of an unidentified germline VH region gene is truly responsible for at least some of the somatic diversification of these antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.