ArticlePlant biotechnology (Tokyo, Japan)2016
Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance.
Article in Plant biotechnology (Tokyo, Japan), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species.Plant, cell & environment · 2026Review
- Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf.Frontiers in plant science · 2022Article
- Nanoparticles for protein delivery in planta.Current opinion in plant biology · 2021Review
- CRISPR ribonucleoprotein-mediated genetic engineering in plants.Plant communications · 2021Review
- A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall.Scientific reports · 2019Article
- Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides.PloS one · 2019Article
- Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent developments of transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) have expanded plant breeding technology. One technical issue related to the current genome editing process is residual transgenes for TALEN and CRISPR/Cas9 left in plant genomes after the editing process. Here, we aim to add transient kanamycin resistance into apple leaf cells by introducing neomycin phosphotransferase II (NPTII) into apple leaf cells using the fusion peptide system. At 75 mg/L of kanamycin for 2 days, apple JM1 leaf cells infiltrated with NPTII could be selected. Thus, we successfully demonstrated the first transient selection system of plant cells using a fusion peptide-mediated protein delivery system.
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Registered trials
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