Evidence map›Paper›PMID 31273307›Full record

ArticleScientific reports2019

Characterization of bacterial communities in wastewater with enhanced taxonomic resolution by full-length 16S rRNA sequencing.

Daniela Numberger, Lars Ganzert, Luca Zoccarato, Kristin Mühldorfer, Sascha Sauer, Hans-Peter Grossart, Alex D Greenwood

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed.

  1. Article
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  7. Predicting Strain-Specific Metabolic Capabilities in the GenusComputational and structural biotechnology journal · 2026
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23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Daniela NumbergerLeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.
Lars GanzertLeibniz Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775, Stechlin, Germany.ORCID http://orcid.org/0000-0001-9595-1041
Luca ZoccaratoLeibniz Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775, Stechlin, Germany.
Kristin MühldorferLeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.
Sascha SauerMax Delbrück Center for Molecular Medicine in the Helmholtz Association (BIMSB/BIH), Robert-Rössle-Strasse 10, 13092, Berlin, Germany.
Hans-Peter GrossartLeibniz Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775, Stechlin, Germany.ORCID http://orcid.org/0000-0002-9141-0325
Alex D GreenwoodLeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315, Berlin, Germany. greenwood@izw-berlin.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Wastewater treatment is crucial to environmental hygiene in urban environments. However, wastewater treatment plants (WWTPs) collect chemicals, organic matter, and microorganisms including pathogens and multi-resistant bacteria from various sources which may be potentially released into the environment via WWTP effluent. To better understand microbial dynamics in WWTPs, we characterized and compared the bacterial community of the inflow and effluent of a WWTP in Berlin, Germany using full-length 16S rRNA gene sequences, which allowed for species level determination in many cases and generally resolved bacterial taxa. Significantly distinct bacterial communities were identified in the wastewater inflow and effluent samples. Dominant operational taxonomic units (OTUs) varied both temporally and spatially. Disease associated bacterial groups were efficiently reduced in their relative abundance from the effluent by the WWTP treatment process, except for Legionella and Leptospira species which demonstrated an increase in relative proportion from inflow to effluent. This indicates that WWTPs, while effective against enteric bacteria, may enrich and release other potentially pathogenic bacteria into the environment. The taxonomic resolution of full-length 16S rRNA genes allows for improved characterization of potential pathogenic taxa and other harmful bacteria which is required to reliably assess health risk.

Indexed as

BacteriaPhylogenyRNA, Ribosomal, 16SWastewaterRNA, Ribosomal, 16SWastewater

Identifiers

PMID31273307
PMCPMC6609626

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.