Evidence map›Paper›PMID 31273250›Full record

ArticleScientific reports2019

Associations between serum and plasma brain-derived neurotrophic factor and influence of storage time and centrifugation strategy.

Anne Kær Gejl, Christian Enevold, Anna Bugge, Marianne Skovsager Andersen, Claus Henrik Nielsen, Lars Bo Andersen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers, 9 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
106citing papers in PubMed, 9 pooled it
7.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

106 citing papers in PubMed, 9 syntheses or guidelines pooled it, 165 citations in OpenAlex.

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  13. Nutritional state-dependent changes in subcortical limbic structures in anorexia nervosa are linked to leptin, ghrelin, and neurofilament light.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026
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46 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Anne Kær GejlUniversity of Southern Denmark, Department of Sports Science and Clinical Biomechanics, Exercise Epidemiology, Odense, Denmark. annekaerg@gmail.com.ORCID http://orcid.org/0000-0001-6781-2263
Christian EnevoldCopenhagen University Hospital Rigshospitalet, Institute for Inflammation Research, Center for Rheumatology and Spine Diseases, Copenhagen, Denmark.
Anna BuggeUniversity College Copenhagen, Faculty of Health, School of Physiotherapy, Copenhagen, Denmark.
Marianne Skovsager AndersenOdense University Hospital, Department of Endocrinology, University of Southern Denmark, Odense, Denmark.
Claus Henrik NielsenCopenhagen University Hospital Rigshospitalet, Institute for Inflammation Research, Center for Rheumatology and Spine Diseases, Copenhagen, Denmark.
Lars Bo AndersenWestern Norway University of Applied Sciences, Faculty of Education, Arts and Sport, Campus Sogndal, Bergen, Norway.
Copenhagen University Hospital · DKUniversity of Southern Denmark · DKUniversity College Copenhagen · DKWestern Norway University of Applied Sciences · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aims of the study were to clarify the impact of storage time and centrifugation strategy on brain-derived neurotrophic factor (BDNF) levels in human serum and plasma samples. In addition, we analyzed associations between BDNF levels, cardiorespiratory fitness and waist circumference. Seventeen healthy males (25.2 (4.1) years) were included in the study. Blood samples were drawn after an overnight fast and treated to different protocols, varying in time before centrifugation and centrifugation strategy. BDNF was analyzed in serum, normal plasma (NP) and platelet-poor plasma (PPP). Also, waist circumference and cardiorespiratory fitness were measured. A large increase was observed in serum BDNF levels during the first hour of clotting. BDNF in NP correlated with PPP, whereas no correlations were found between BDNF in serum and plasma. Though not statistical significant, correlations between fitness and BDNF in serum changed from positive at 30 min. to negative when clotting time was ≥60 min. In conclusion, BDNF levels in serum were affected by clotting time, whereas BDNF levels in plasma were influenced by centrifugation strategy. Importantly, BDNF in serum and plasma appears to reflect two different pools of BDNF. The biological relevance of the velocity of BDNF release during clotting and its dependence upon fitness must be investigated further.

Indexed as

Cardiorespiratory FitnessAdultBlood Specimen CollectionBrain-Derived Neurotrophic FactorCentrifugationHumansMalePlasmaSerumTime FactorsWaist CircumferenceBDNF protein, humanBrain-Derived Neurotrophic Factor

Identifiers

PMID31273250
PMCPMC6609657
OpenAlexW2955047360

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.