ArticleBMC genomics2019
The haustorial transcriptome of the cucurbit pathogen Podosphaera xanthii reveals new insights into the biotrophy and pathogenesis of powdery mildew fungi.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Cracking the wall: the fungal cell wall assembly protein ECM33 is a promising molecular target in powdery mildew fungi.Horticulture research · 2026Article
- Machine Learning- and AI-Driven QSAR Models for the Discovery of Novel Potential Fungicides: FAPI (Fungicide Targeting Acid Phosphatase Inhibition).Journal of agricultural and food chemistry · 2026Article
- Discovering the dynamics of peach fruit mycobiome throughout fruit development season by high-throughput sequencing.Scientific reports · 2025Article
- Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein.PLoS pathogens · 2024Article
- Suppression of Chitin-Triggered Immunity by Plant Fungal Pathogens: A Case Study of the Cucurbit Powdery Mildew FungusJournal of fungi (Basel, Switzerland) · 2023Review
- Suppression of Chitin-Triggered Immunity by a New Fungal Chitin-Binding Effector Resulting from Alternative Splicing of a Chitin Deacetylase Gene.Journal of fungi (Basel, Switzerland) · 2022Article
- GWAS Reveals a Novel Candidate GeneInternational journal of molecular sciences · 2022Article
- The haustorium: The root of biotrophic fungal pathogens.Frontiers in plant science · 2022Review
- Review
- Chitin Deacetylase, a Novel Target for the Design of Agricultural Fungicides.Journal of fungi (Basel, Switzerland) · 2021Article
- Gene Mining for Conserved, Non-Annotated Proteins ofJournal of fungi (Basel, Switzerland) · 2021Article
- A haustorial-expressed lytic polysaccharide monooxygenase from the cucurbit powdery mildew pathogen Podosphaera xanthii contributes to the suppression of chitin-triggered immunity.Molecular plant pathology · 2021Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundPodosphaera xanthii is the main causal agent of powdery mildew disease in cucurbits and is responsible for important yield losses in these crops worldwide. Powdery mildew fungi are obligate biotrophs. In these parasites, biotrophy is determined by the presence of haustoria, which are specialized structures of parasitism developed by these fungi for the acquisition of nutrients and the delivery of effectors. Detailed molecular studies of powdery mildew haustoria are scarce due mainly to difficulties in their isolation. Therefore, their analysis is considered an important challenge for powdery mildew research. The aim of this work was to gain insights into powdery mildew biology by analysing the haustorial transcriptome of P. xanthii.
resultsPrior to RNA isolation and massive-scale mRNA sequencing, a flow cytometric approach was developed to isolate P. xanthii haustoria free of visible contaminants. Next, several commercial kits were used to isolate total RNA and to construct the cDNA and Illumina libraries that were finally sequenced by the Illumina NextSeq system. Using this approach, the maximum amount of information from low-quality RNA that could be obtained was used to accomplish the de novo assembly of the P. xanthii haustorial transcriptome. The subsequent analysis of this transcriptome and comparison with the epiphytic transcriptome allowed us to identify the importance of several biological processes for haustorial cells such as protection against reactive oxygen species, the acquisition of different nutrients and genetic regulation mediated by non-coding RNAs. In addition, we could also identify several secreted proteins expressed exclusively in haustoria such as cell adhesion proteins that have not been related to powdery mildew biology to date.
conclusionsThis work provides a novel approach to study the molecular aspects of powdery mildew haustoria. In addition, the results of this study have also allowed us to identify certain previously unknown processes and proteins involved in the biology of powdery mildews that could be essential for their biotrophy and pathogenesis.
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