Evidence map›Paper›PMID 31271857›Full record

ArticleBiomaterials2019

Physical confinement induces malignant transformation in mammary epithelial cells.

Yen-Chun Lu, Tinyi Chu, Matthew S Hall, Dah-Jiun Fu, Quanming Shi, Alan Chiu, Duo An, Long-Hai Wang, Yehudah Pardo, Teresa Southard and 7 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 2 pooled it
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 20 citations in OpenAlex.

  1. Pooled it
  2. 3DACS biomaterials science & engineering · 2023
    Pooled it
  3. Review
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  5. Review
  6. Review
  7. Article
  8. Article
  9. Review
  10. Review
  11. Obesity, sleep apnea, and cancer.International journal of obesity (2005) · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 2 institutions in 1 country.

Yen-Chun LuDepartment of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY, 14853, USA. Electronic address: yl2347@cornell.edu.
Tinyi ChuBaker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14853, USA.
Matthew S HallDepartment of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY, 14853, USA.
Dah-Jiun FuDepartment of Biomedical Science, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca, NY, 14853, USA.
Quanming ShiBioengineering, Stanford University, 443 Via Ortega Shriram Center, Stanford, CA, 94305, USA; BioX Institute, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA.
Alan ChiuDepartment of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY, 14853, USA.
Duo AnDepartment of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY, 14853, USA.
Long-Hai WangDepartment of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY, 14853, USA.
Yehudah PardoNancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA.
Teresa SouthardDepartment of Biomedical Science, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca, NY, 14853, USA.
Charles G DankoBaker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14853, USA; Department of Biomedical Science, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca, NY, 14853, USA.
Jan LiphardtBioengineering, Stanford University, 443 Via Ortega Shriram Center, Stanford, CA, 94305, USA; BioX Institute, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA.
Alexander Yu NikitinDepartment of Biomedical Science, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca, NY, 14853, USA.
Mingming WuDepartment of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY, 14853, USA.
Claudia FischbachNancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA; Kavli Institute at Cornell for Nanoscale Science, Cornell University, Ithaca, NY, 14853, USA.
Scott CoonrodBaker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14853, USA; Department of Biomedical Science, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca, NY, 14853, USA. Electronic address: sac269@cornell.edu.
Minglin MaDepartment of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY, 14853, USA. Electronic address: mm826@cornell.edu.
Cornell University · USStanford University · US

Funding

Small RNA Pathways in Mammalian GametogenesisP50HD076210 · NICHD · CORNELL UNIVERSITY · PI SCHLEGEL, PETER N · 2014 to 2018
$7.4M
Microfluidic platform for tumor cell invasionR01CA221346 · NCI · CORNELL UNIVERSITY · PI Mingming Wu · 2017 to 2026
$3.4M
Origins of Ovarian CarcinomaR01CA182413 · NCI · CORNELL UNIVERSITY · PI NIKITIN, ALEXANDER Y. · 2015 to 2019
$1.8M
Neuroendocrine mechanisms of prostate cancer progressionR01CA197160 · NCI · CORNELL UNIVERSITY · PI NIKITIN, ALEXANDER Y. · 2015 to 2020
$1.8M
Zeiss LSM710 Confocal Microscope for Shared Imaging FacilityS10RR025502 · NCRR · CORNELL UNIVERSITY · PI WILLIAMS, REBECCA M · 2009 to 2009
$500k
Organogenesis in microcapsules: developing an efficient and scalable organoid culture platformR03EB020910 · NIBIB · CORNELL UNIVERSITY · PI MA, MINGLIN · 2015 to 2016
$144k
NCI NIH HHS R01 CA182413NCI NIH HHS R01 CA197160NCI NIH HHS R01 CA221346NCRR NIH HHS S10 RR025502NIBIB NIH HHS R03 EB020910NICHD NIH HHS P50 HD076210
6 · The paper itself

Abstract

The physical microenvironment of tumor cells plays an important role in cancer initiation and progression. Here, we present evidence that confinement - a new physical parameter that is apart from matrix stiffness - can also induce malignant transformation in mammary epithelial cells. We discovered that MCF10A cells, a benign mammary cell line that forms growth-arrested polarized acini in Matrigel, transforms into cancer-like cells within the same Matrigel material following confinement in alginate shell hydrogel microcapsules. The confined cells exhibited a range of tumor-like behaviors, including uncontrolled cellular proliferation and invasion. Additionally, 4-6 weeks after transplantation into the mammary fad pads of immunocompromised mice, the confined cells formed large palpable masses that exhibited histological features similar to that of carcinomas. Taken together, our findings suggest that physical confinement represents a previously unrecognized mechanism for malignancy induction in mammary epithelial cells and also provide a new, microcapsule-based, high throughput model system for testing new breast cancer therapeutics.

Indexed as

Acinar CellsAnimalsCapsulesCarcinogenesisCell Transformation, NeoplasticEpithelial CellsExtracellular MatrixFemaleHumansHydrogelsInsulinInsulin-Like Growth Factor IMammary Glands, HumanMice, SCIDSequence Analysis, RNASignal TransductionCapsulesHydrogelsInsulinInsulin-Like Growth Factor IBreast cancerHydrogelPhysical confinementTumorigenesis

Identifiers

PMID31271857
PMCPMC7336132
OpenAlexW2955219521

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.