ArticleJournal of cellular physiology2020
Hippuric acid and 3-(3-hydroxyphenyl) propionic acid inhibit murine osteoclastogenesis through RANKL-RANK independent pathway.
Article in Journal of cellular physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Moderate consumption of freeze-dried blueberry powder increased net bone calcium retention compared with no treatment in healthy postmenopausal women: a randomized crossover trial.The American journal of clinical nutrition · 2023Trial
- Targeting the gut-bone axis in osteoporosis: probiotic interventions and other microbiota-based therapeutic strategies.Gut microbes · 2026Review
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- Serum metabolic fingerprinting for diagnosis and therapeutic applications of ovarian endometriosis.iScience · 2026Article
- Therapeutic Effects of the Most Common Polyphenols Found inNutrients · 2026Review
- Dietary Tart Cherry and Fructooligosaccharides Promote Bone Health via the Gut Microbiota and Increased Bone Formation.Nutrients · 2025Article
- Sex dependent effects of GPR109A gene deletion in myeloid cells on bone development in mice.Scientific reports · 2025Article
- Microbes Matter: Exploring the Connection Between Infant Gut Microbiota and Bone Development.Calcified tissue international · 2025Article
- Classifying patients with psoriatic arthritis according to their disease activity status using serum metabolites and machine learning.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Article
- Interplay between Phytochemicals and the Colonic Microbiota.Nutrients · 2023Review
- Nox4 expression in osteo-progenitors controls bone development in mice during early life.Communications biology · 2022Article
- In Vitro Intestinal Bioaccessibility and Colonic Biotransformation of Polyphenols from Mini Bell Peppers (Capsicum annuum L.).Plant foods for human nutrition (Dordrecht, Netherlands) · 2022Article
- Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy.Bioactive materials · 2022Article
- Gut microbiota-derived metabolites in inflammatory diseases based on targeted metabolomics.Frontiers in pharmacology · 2022Article
- Integration of the Human Gut Microbiome and Serum Metabolome Reveals Novel Biological Factors Involved in the Regulation of Bone Mineral Density.Frontiers in cellular and infection microbiology · 2022Article
- Short-Term Increased Physical Activity During Early Life Affects High-Fat Diet-Induced Bone Loss in Young Adult Mice.JBMR plus · 2021Article
- Dried plum consumption improves bone mineral density in osteopenic postmenopausal woman: A case report.Bone reports · 2021Article
- Bone Marrow Homeostasis Is Impaired via JAK/STAT and Glucocorticoid Signaling in Cancer Cachexia Model.Cancers · 2021Article
- GPR109A mediates the effects of hippuric acid on regulating osteoclastogenesis and bone resorption in mice.Communications biology · 2021Article
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3 authors.
Funding
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Abstract
Nutritional factors influence bone development. Previous studies demonstrated that bone mass significantly increased with suppressed bone resorption in early life of rats fed with AIN-93G semi-purified diets supplemented with 10% whole blueberry (BB) powder for 2 weeks. However, the effects of increased phenolic acids in animal serum due to this diet on bone and bone resorption were unclear. This in vitro and in ex vivo study examined the effects of phenolic hippuric acid (HA) and 3-(3-hydroxyphenyl) propionic acid (3-3-PPA) on osteoclastic cell differentiation and bone resorption. We cultured murine osteoclast (macrophage) cell line, RAW 264.7 cells, and hematopoietic osteoclast progenitor cells (isolated from 4-week-old C57BL6/J mice) with 50 ng/ml of receptor activator of nuclear factor κ-Β ligand (RANKL). Morphologic studies showed decreased osteoclast number with treatment of 2.5% mouse serum from BB diet-fed animals compared with those treated with serum from standard casein diet-fed mice in both RAW 264.7 cell and primary cell cultures. HA and 3-3-PPA, but not 3-4-PPA, had dose-dependent suppressive effects on osteoclastogenesis and osteoclast resorptive activity in Corning osteo-assay plates. Signaling pathway analysis showed that after pretreatment with HA or 3-3-PPA, RANKL-stimulated increase of osteoclastogenic markers, such as nuclear factor of activated T-cells, cytoplasmic 1 and matrix metallopeptidase 9 gene/protein expression were blunted. Inhibitory effects of HA and 3-3-PPA on osteoclastogenesis utilized RANKL/RANK independent mediators. The study revealed that HA and 3-3-PPA significantly inhibited osteoclastogenesis and bone osteoclastic resorptive activity.
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