Evidence map›Paper›PMID 31270459›Full record

ArticleNature2019

Single-cell analysis reveals T cell infiltration in old neurogenic niches.

Ben W Dulken, Matthew T Buckley, Paloma Navarro Negredo, Naresha Saligrama, Romain Cayrol, Dena S Leeman, Benson M George, Stéphane C Boutet, Katja Hebestreit, John V Pluvinage and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 327 papers.

0numbers the graph read from it
0cells of the map it votes in
327citing papers in PubMed
28.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

327 citing papers in PubMed, 541 citations in OpenAlex.

  1. Review
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267 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Ben W Dulken *Department of Genetics, Stanford University, Stanford, CA, USA.
Matthew T Buckley *Department of Genetics, Stanford University, Stanford, CA, USA.
Paloma Navarro Negredo *Department of Genetics, Stanford University, Stanford, CA, USA.
Naresha SaligramaDepartment of Immunology and Microbiology, Stanford University, Stanford, CA, USA.
Romain CayrolDepartment of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
Dena S LeemanDepartment of Genetics, Stanford University, Stanford, CA, USA.
Benson M GeorgeStanford Medical Scientist Training Program, Stanford University, Stanford, CA, USA.
Stéphane C BoutetFluidigm Corporation, South San Francisco, CA, USA.
Katja HebestreitDepartment of Genetics, Stanford University, Stanford, CA, USA.
John V PluvinageStanford Medical Scientist Training Program, Stanford University, Stanford, CA, USA.
Tony Wyss-CorayDepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA.
Irving L WeissmanInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
Hannes VogelDepartment of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
Mark M DavisDepartment of Immunology and Microbiology, Stanford University, Stanford, CA, USA.
Anne BrunetDepartment of Genetics, Stanford University, Stanford, CA, USA. abrunet1@stanford.edu.
Stanford University · USCalifornia Institute for Regenerative Medicine · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007365 · NIGMS · STANFORD UNIVERSITY · PI CHUA, KATRIN F · 1985 to 2021
$33.8M
INSTITUTIONAL TRAINING GRANT IN GENOME SCIENCET32HG000044 · NHGRI · STANFORD UNIVERSITY · PI MICHAEL P. SNYDER · 1995 to 2026
$32.2M
Wnt signaling in muscle stem cell agingP01AG036695 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MARGARET A. GOODELL · 2011 to 2026
$29.3M
Stanford Training Program in Aging ResearchT32AG047126 · NIA · STANFORD UNIVERSITY · PI VYJEYANTHI S PERIYAKOIL, TONY WYSS-CORAY · 2014 to 2026
$4.3M
NHGRI NIH HHS T32 HG000044NIA NIH HHS P01 AG036695NIA NIH HHS T32 AG047126NIGMS NIH HHS T32 GM007365
6 · The paper itself

Abstract

The mammalian brain contains neurogenic niches that comprise neural stem cells and other cell types. Neurogenic niches become less functional with age, but how they change during ageing remains unclear. Here we perform single-cell RNA sequencing of young and old neurogenic niches in mice. The analysis of 14,685 single-cell transcriptomes reveals a decrease in activated neural stem cells, changes in endothelial cells and microglia, and an infiltration of T cells in old neurogenic niches. T cells in old brains are clonally expanded and are generally distinct from those in old blood, which suggests that they may experience specific antigens. T cells in old brains also express interferon-γ, and the subset of neural stem cells that has a high interferon response shows decreased proliferation in vivo. We find that T cells can inhibit the proliferation of neural stem cells in co-cultures and in vivo, in part by secreting interferon-γ. Our study reveals an interaction between T cells and neural stem cells in old brains, opening potential avenues through which to counteract age-related decline in brain function.

Indexed as

Cell MovementNeurogenesisSingle-Cell AnalysisAgingAnimalsBloodBrainCell ProliferationClone CellsCoculture TechniquesEndothelial CellsInterferon-gammaMaleMiceMice, Inbred C57BLMicrogliaInterferon-gamma

Identifiers

PMID31270459
PMCPMC7111535
OpenAlexW2954434934

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.