ArticleAnaerobe2019
A novel conjugal donor strain for improved DNA transfer into Clostridium spp.
Article in Anaerobe, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Generating Targeted Mutants Using a Riboswitch to Control Plasmid Behavior During Allelic Exchange Mutagenesis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optimization of Plasmid Curing from Genetically EngineeredACS synthetic biology · 2025Article
- Expression of Recombinant Clostridial Neurotoxin byMicroorganisms · 2024Article
- Formation of the pyruvoyl-dependent proline reductase Prd fromPNAS nexus · 2024Article
- Development of a genetic engineering toolbox for syngas-utilizing acetogen Clostridium sp. AWRP.Microbial cell factories · 2024Article
- Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals.Frontiers in bioengineering and biotechnology · 2024Review
- The Restriction-Modification Systems ofMicroorganisms · 2023Article
- Genetic Engineering of Resident Bacteria in the Gut Microbiome.Journal of bacteriology · 2023Review
- Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.Biotechnology for biofuels and bioproducts · 2023Article
- Endogenous CRISPR/Cas systems for genome engineering in the acetogensFrontiers in bioengineering and biotechnology · 2023Article
- Refined control of CRISPR-Cas9 gene editing inFrontiers in immunology · 2023Article
- Base editing enables duplex point mutagenesis inFrontiers in bioengineering and biotechnology · 2023Article
- Deletion of genes linked to the CFrontiers in bioengineering and biotechnology · 2023Article
- Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO.Microbial cell factories · 2022Article
- Barriers to genetic manipulation of Enterococci: Current Approaches and Future Directions.FEMS microbiology reviews · 2022Review
- Design, Analysis, and Implementation of a Novel Biochemical Pathway for Ethylene Glycol Production inACS synthetic biology · 2022Article
- Towards Development of a Non-ToxigenicPharmaceutics · 2022Article
- Required Gene Set for Autotrophic Growth ofApplied and environmental microbiology · 2022Article
- Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences.Nucleic acids research · 2022Article
- Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiome.Cell · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Clostridium encompasses species which are relevant to human and animal disease as well as species which have industrial potential, for instance, as producers of chemicals and fuels or as tumour delivery vehicles. Genetic manipulation of these target organisms is critical for advances in these fields. DNA transfer efficiencies, however, vary between species. Low efficiencies can impede the progress of research efforts. A novel conjugal donor strain of Escherichia coli has been created which exhibits a greater than 10-fold increases in conjugation efficiency compared to the traditionally used CA434 strain in the three species tested; C. autoethanogenum DSM 10061, C. sporogenes NCIMB 10696 and C. difficile R20291. The novel strain, designated 'sExpress', does not methylate DNA at Dcm sites (CCWGG) which allows circumvention of cytosine-specific Type IV restriction systems. A robust protocol for conjugation is presented which routinely produces in the order of 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.