ArticleThe EMBO journal2019
Genome-scale, single-cell-type resolution of microRNA activities within a whole plant organ.
Article in The EMBO journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 60 citations in OpenAlex.
- Three decades of mobile RNA silencing within plants: what have we learnt?Journal of experimental botany · 2026Review
- Transcriptome-wide analysis of Arabidopsis DICER-LIKE1 RNA substrates.Nucleic acids research · 2026Article
- Foliar-applied double-stranded RNA is mobile, transfers to plant pathogens, and triggers RNAi.Nucleic acids research · 2026Article
- The nuclear pore complex acts as a hub for pri-miRNA transcription and processing in plants.Nucleic acids research · 2025Article
- Exploring the untapped potential of single-cell and spatial omics in plant biology.The New phytologist · 2025Review
- A pectin acetyl-transferase facilitates secondary plasmodesmata formation and RNA silencing movement between plant cells.The Plant journal : for cell and molecular biology · 2025Article
- A pipeline for validation of Serendipita indica effector-like sRNA suggests cross-kingdom communication in the symbiosis with Arabidopsis.Journal of experimental botany · 2025Article
- MicroRNA gatekeepers: Orchestrating rhizospheric dynamics.Journal of integrative plant biology · 2025Review
- RNA interference shapes stress responses inFrontiers in plant science · 2025Review
- Microinjection of the CRISPR/Cas9 editing system through the germ pore of a wheat microspore induces mutations in the target Ms2 gene.Molecular biology reports · 2024Article
- An AGO10:miR165/6 module regulates meristem activity and xylem development in the Arabidopsis root.The EMBO journal · 2024Article
- Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023Review
- Small RNA-mediated DNA methylation during plant reproduction.The Plant cell · 2023Review
- Recent Insights into Plant miRNA Biogenesis: Multiple Layers of miRNA Level Regulation.Plants (Basel, Switzerland) · 2023Review
- A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19.The Plant journal : for cell and molecular biology · 2023Article
- Plant gene silencing signals move from the phloem to influence gene expression in shoot apical meristems.BMC plant biology · 2022Article
- Profiling of Single-cell-type-specific MicroRNAs inBio-protocol · 2022Article
- Suppression of both intra- and intercellular RNA silencing by the tombusviral P19 protein requires its small RNA binding property.The New phytologist · 2022Article
- Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis.Developmental cell · 2022Article
- R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants.Nature plants · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Loaded into ARGONAUTE(AGO) proteins, eukaryotic micro(mi)RNAs regulate gene expression via cleavage, translational repression, and/or accelerated decay of sequence-complementary target transcripts. Despite their importance in development, cell identity maintenance and stress responses, how individual miRNAs contribute to spatial gene regulation within the complex cell mosaics formed in tissues/organs has remained inaccessible in any organism to date. We have developed a non-invasive methodology to examine, at single-cell-type resolution, the AGO-loading and activity patterns of entire miRNA cohorts in intact organs, applied here to the Arabidopsis root tip. A dual miRNAome-targetome analytical interface allowing intuitive data integration/visualization was developed as the basis for in-depth investigations via single-cell-type experimentation. These uncovered an array of so far speculative or hitherto unknown types of spatial miRNA-mediated gene regulation schemes, including via widespread cell-to-cell movement between contiguous layers of distinct identities. This study provides the proof of principle that minimally invasive, genome-scale analysis of miRNA activities within and between single-cell types of whole organs is achievable.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.