ArticleThe EMBO journal2019
Identification of a basal system for unwinding a bacterial chromosome origin.
Article in The EMBO journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria.Microbiology (Reading, England) · 2025Article
- Article
- The bacterial replication origin BUS promotes nucleobase capture.Nature communications · 2023Article
- IHF and Fis asInternational journal of molecular sciences · 2023Review
- Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.Nucleic acids research · 2023Article
- SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation.Nucleic acids research · 2023Article
- The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin.Nucleic acids research · 2023Article
- Toward an understanding of the DNA replication initiation in bacteria.Frontiers in microbiology · 2023Review
- Dynamics of chromosome organization in a minimal bacterial cell.Frontiers in cell and developmental biology · 2023Article
- Ori-Finder 2022: A Comprehensive Web Server for Prediction and Analysis of Bacterial Replication Origins.Genomics, proteomics & bioinformatics · 2022Article
- HU Knew? Bacillus subtilis HBsu Is Required for DNA Replication Initiation.Journal of bacteriology · 2022Article
- Evidence for a chromosome origin unwinding system broadly conserved in bacteria.Nucleic acids research · 2021Article
- Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif uponInternational journal of molecular sciences · 2021Article
- Characterization of ori and parS-like functions in secondary genome replicons inLife science alliance · 2021Article
- Interactions of replication initiator RctB with single- and double-stranded DNA in origin opening of Vibrio cholerae chromosome 2.Nucleic acids research · 2020Article
- Identification of the Unwinding Region in theFrontiers in microbiology · 2020Article
- Identification of a basal system for unwinding a bacterial chromosome origin.The EMBO journal · 2019Article
- Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.Frontiers in microbiology · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Genome duplication is essential for cell proliferation, and DNA synthesis is generally initiated by dedicated replication proteins at specific loci termed origins. In bacteria, the master initiator DnaA binds the chromosome origin (oriC) and unwinds the DNA duplex to permit helicase loading. However, despite decades of research it remained unclear how the information encoded within oriC guides DnaA-dependent strand separation. To address this fundamental question, we took a systematic genetic approach in vivo and identified the core set of essential sequence elements within the Bacillus subtilis chromosome origin unwinding region. Using this information, we then show in vitro that the minimal replication origin sequence elements are necessary and sufficient to promote the mechanical functions of DNA duplex unwinding by DnaA. Because the basal DNA unwinding system characterized here appears to be conserved throughout the bacterial domain, this discovery provides a framework for understanding oriC architecture, activity, regulation and diversity.
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