ArticleJournal of assisted reproduction and genetics2019
Unstable osmolality of microdrops cultured in non-humidified incubators.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Impact of mineral oil overlay on immature oocyte maturation in an optimized in vitro culture system.European journal of medical research · 2026Article
- Reactive Oxygen Species in Embryo Development: Sources, Impacts, and Implications for In Vitro Culture Systems.Life (Basel, Switzerland) · 2026Review
- High-viscosity oil does not offer enhanced protection for osmolality changes in high-volume media drops during extended dry incubation.Journal of assisted reproduction and genetics · 2025Article
- In Vitro Maturation and Time-Lapse Imaging of Immature Human Oocytes: A Direction Toward Clinical Applications.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development and application of vibrating dynamic culture system for mouse oocytes and embryos.Frontiers in veterinary science · 2025Article
- The Task Matters: A Comprehensive Review and Proposed Literature Score of the Effects of Chemical and Physical Parameters on Embryo Developmental Competence.Life (Basel, Switzerland) · 2023Review
- Evaluation of an automated dish preparation system for IVF and embryo culture using a mouse mode.Scientific reports · 2023Article
- Humidified atmosphere in a time-lapse embryo culture system does not improve ongoing pregnancy rate: a retrospective propensity score model study derived from 496 first ICSI cycles.Journal of assisted reproduction and genetics · 2023Article
- How to optimize culture media osmolality during Assisted Reproductive Technologies treatments.JBRA assisted reproduction · 2023Observational
- Effects of oxygen tension and humidity on the preimplantation development of mouse embryos produced by in vitro fertilization: analysis using a non-humidifying incubator with time-lapse cinematography.Experimental animals · 2022Article
- Use of mineral oil in IVF culture systems: physico-chemical aspects, management, and safety.Journal of assisted reproduction and genetics · 2022Review
- Oxidative Stress and Assisted Reproduction: A Comprehensive Review of Its Pathophysiological Role and Strategies for Optimizing Embryo Culture Environment.Antioxidants (Basel, Switzerland) · 2022Review
- Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.F1000Research · 2022Article
- Back to the future: optimised microwell culture of individual human preimplantation stage embryos.Journal of assisted reproduction and genetics · 2021Article
- Good practice recommendations for the use of time-lapse technologyHuman reproduction open · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the stability of osmolality in non-humidified and humidified incubators for assisted reproductive technologies (ART).
methodsDrops of three single-step culture media (media A, B, and C) were incubated for 5 or 6 days covered with four different mineral oils (oils A, B, C, and D) in non-humidified incubator A, non-humidified incubator B, or humidified incubator C to investigate the effects of incubator environment (humidification), drop volume, culture media, and mineral oil on the stability of osmolality in microdrops.
resultsA significant and linear increase was shown in the osmolality of 50-μL and 200-μL microdrops covered with mineral oil during 5 days incubation in non-humidified benchtop incubators. The maximum increase was 20 mOsm/kg, and the extent of the increase was affected by microdrop volume and possibly by the type of mineral oil used to cover the drops. In contrast, the osmolality of 50-μL and 200-μL microdrops did not change during 5 days incubation in a humidified benchtop incubator.
conclusionsMineral oil alone may not adequately prevent gradual changes in the osmolality of low-volume microdrops during extended in vitro culture of human embryos in non-humidified incubators. As a result, the osmolality may increase to high enough levels to stress some human embryos and adversely affect clinical outcomes. We therefore recommend that the stability of osmolality should be given more consideration to ensure optimal culture conditions for ART.
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