ArticleProceedings of the National Academy of Sciences of the United States of America2019
High-frequency microrheology in 3D reveals mismatch between cytoskeletal and extracellular matrix mechanics.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 69 citations in OpenAlex.
- Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential.Advanced healthcare materials · 2026Review
- Matrix stress relaxation drives glioblastoma cell response in viscoelastic biomaterials.Science advances · 2026Article
- Massart iron oxide nanoparticles in mechanobiology.Nanoscale advances · 2026Review
- Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy.Research square · 2026Article
- Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy.bioRxiv : the preprint server for biology · 2026Article
- High-frequency micro-viscoelasticity of biofluids by using optical trapping interferometry.Biophysical reviews · 2026Review
- Engineered bone matrix models for understanding breast cancer skeletal metastasis.Cancer metastasis reviews · 2026Review
- Macrophages Mediate Mesoscale Brain Mechanical Homeostasis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Characteristic frequencies of localized stress relaxation in scaling-law rheology of living cells.Biophysical journal · 2025Article
- The role of dynamic reciprocity in 3D cell migration: connecting cell and matrix mechanics to migratory plasticity.npj biological physics and mechanics · 2025Review
- Length and time scales bridge poroelasticity and tissue architecture.Journal of biosciences · 2025Article
- Cellular Energy Cycle Mediates an Advection-Like Forward Cell Flow to Support Collective Invasion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Laser speckle rheological microscopy reveals wideband viscoelastic spectra of biological tissues.Science advances · 2024Article
- Macrophage mediated mesoscale brain mechanical homeostasis mechanically imaged via optical tweezers and Brillouin microscopybioRxiv : the preprint server for biology · 2024Article
- Scaling-law mechanical marker for liver fibrosis diagnosis and drug screening through machine learning.Frontiers in bioengineering and biotechnology · 2024Article
- Cancer cell response to extrinsic and intrinsic mechanical cue: opportunities for tumor apoptosis strategies.Regenerative biomaterials · 2024Review
- YAP localization mediates mechanical adaptation of human cancer cells during extravasationbioRxiv : the preprint server for biology · 2023Article
- S-Protected Thiolated Chitosan versus Thiolated Chitosan as Cell Adhesive Biomaterials for Tissue Engineering.ACS applied materials & interfaces · 2023Article
- The extracellular matrix mechanics in the vasculature.Nature cardiovascular research · 2023Review
- Vascular regulation of disseminated tumor cells during metastatic spread.Biophysics reviews · 2023Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mechanical homeostasis describes how cells sense physical cues from the microenvironment and concomitantly remodel both the cytoskeleton and the surrounding extracellular matrix (ECM). Such feedback is thought to be essential to healthy development and maintenance of tissue. However, the nature of the dynamic coupling between microscale cell and ECM mechanics remains poorly understood. Here we investigate how and whether cells remodel their cortex and basement membrane to adapt to their microenvironment. We measured both intracellular and extracellular viscoelasticity, generating a full factorial dataset on 5 cell lines in 2 ECMs subjected to 4 cytoskeletal drug treatments at 2 time points. Nonmalignant breast epithelial cells show a similar viscoelasticity to that measured for the local ECM when cultured in 3D laminin-rich ECM. In contrast, the malignant counterpart is stiffer than the local environment. We confirmed that other mammary cancer cells embedded in tissue-mimetic hydrogels are nearly 4-fold stiffer than the surrounding ECM. Perturbation of actomyosin did not yield uniform responses but instead depended on the cell type and chemistry of the hydrogel. The observed viscoelasticity of both ECM and cells were well described by power laws in a frequency range that governs single filament cytoskeletal dynamics. Remarkably, the intracellular and extracellular power law parameters for the entire dataset collectively fall onto 2 parallel master curves described by just 2 parameters. Our work shows that tumor cells are mechanically plastic to adapt to many environments and reveals dynamical scaling behavior in the microscale mechanical responses of both cells and ECM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.