ArticleMicrobial biotechnology2019
Fluorescence-based analysis of the intracytoplasmic membranes of type I methanotrophs.
Article in Microbial biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Membrane properties modulate methane oxidation by particulate methane monooxygenase.The Journal of biological chemistry · 2026Article
- Innate Immune Function of Neutrophil Cytoplasts Generated Post-Vital NETosis.Research square · 2026Article
- Copper requirements and copper toxicity as niche-defining factors in the growth of terrestrial ammonia-oxidizing archaea and bacteria.FEMS microbiology ecology · 2026Article
- Recent findings in methanotrophs: genetics, molecular ecology, and biopotential.Applied microbiology and biotechnology · 2024Review
- From genome to evolution: investigating type II methylotrophs using a pangenomic analysis.mSystems · 2024Article
- Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.Chemical reviews · 2024Review
- A methanotrophic bacterium to enable methane removal for climate mitigation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Intracytoplasmic membranes develop in Geobacter sulfurreducens under thermodynamically limiting conditions.NPJ biofilms and microbiomes · 2023Article
- A Cristae-Like Microcompartment inmBio · 2022Article
- Structure and activity of particulate methane monooxygenase arrays in methanotrophs.Nature communications · 2022Article
- Fluorescence-based analysis of the intracytoplasmic membranes of type I methanotrophs.Microbial biotechnology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most methanotrophic bacteria maintain intracytoplasmic membranes which house the methane-oxidizing enzyme, particulate methane monooxygenase. Previous studies have primarily used transmission electron microscopy or cryo-electron microscopy to look at the structure of these membranes or lipid extraction methods to determine the per cent of cell dry weight composed of lipids. We show an alternative approach using lipophilic membrane probes and other fluorescent dyes to assess the extent of intracytoplasmic membrane formation in living cells. This fluorescence method is sensitive enough to show not only the characteristic shift in intracytoplasmic membrane formation that is present when methanotrophs are grown with or without copper, but also differences in intracytoplasmic membrane levels at intermediate copper concentrations. This technique can also be employed to monitor dynamic intracytoplasmic membrane changes in the same cell in real time under changing growth conditions. We anticipate that this approach will be of use to researchers wishing to visualize intracytoplasmic membranes who may not have access to electron microscopes. It will also have the capability to relate membrane changes in individual living cells to other measurements by fluorescence labelling or other single-cell analysis methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.