ArticleVirchows Archiv : an international journal of pathology2019
Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen.
Article in Virchows Archiv : an international journal of pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- Morphological artifacts in pulmonary pathology and literature review.Virchows Archiv : an international journal of pathology · 2026Review
- Effect of cold ischemia time and storage temperature on anaplastic lymphoma kinase (ALK) expression in surgically resected lung cancer specimens.Translational lung cancer research · 2026Article
- Unveiling Another Dimension: Advanced Visualization of Cancer Invasion and Metastasis via Micro-CT Imaging.Cancers · 2025Article
- Advances in clinical and translational research in endodontics: A comprehensive overview.Brazilian dental journal · 2025Review
- Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue.Animals : an open access journal from MDPI · 2024Article
- Virtual histological staining of unlabeled autopsy tissue.Nature communications · 2024Article
- Digital pathology for nonalcoholic steatohepatitis assessment.Nature reviews. Gastroenterology & hepatology · 2024Review
- Higher densities of T-lymphocytes in the subsynovial connective tissue of people with carpal tunnel syndrome.PloS one · 2024Article
- Coupling imaging mass cytometry with Alcian blue histochemical staining for a single-slide approach.Frontiers in immunology · 2024Article
- Impact of formalin fixation on mismatch repair protein evaluation by immunohistochemistry.Virchows Archiv : an international journal of pathology · 2023Article
- Slide-to-Slide Tissue Transfer and Array Assembly From Limited Samples for Comprehensive Molecular Profiling.Laboratory investigation; a journal of technical methods and pathology · 2023Article
- Epitope Lability of Phosphorylated Biomarkers of the DNA Damage Response Pathway Results in Increased Vulnerability to Effects of Delayed or Incomplete Formalin Fixation.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Pathology of Cholangiocarcinoma.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2023Article
- The Impact of Pre-analytical Quality Initiatives on Cholangiocarcinoma Diagnostics in Thailand.Frontiers in public health · 2022Article
- Recommendations for streamlining precision medicine in breast cancer care in Latin America.Cancer reports (Hoboken, N.J.) · 2021Review
- Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques.Scientific reports · 2021Article
- The impact of pre-analytical parameters on class II biomarkers by immunohistochemistry: concordance across four tissue processing protocols.Virchows Archiv : an international journal of pathology · 2021Article
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13 authors.
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Abstract
Pre-analytical factors, such as fixation time, influence morphology of diagnostic and predictive immunohistochemical staining, which are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer. From lung resections, specimen with tumor size bigger than 4 cm, 10 samples were obtained: 2 were put through the standard fixation protocol, 5 through the delayed, and 3 through the prolonged fixation protocol. After paraffin embedding, tissue microarrays (TMAs) were made. They were stained with 20 antibodies and scored for quality and intensity of staining. Samples with delay in fixation showed loss of TMA cores on glass slides and deterioration of tissue quality leading to reduction in the expression of CK 7, Keratin MNF116, CAM 5.2, CK 5/6, TTF-1, C-MET, Napsin A, D2-40, and PD-L1. Prolonged fixation had no influence on the performance of immunohistochemical stains. Delay of fixation negatively affects the expression of different immunohistochemical markers, influencing diagnostic (cytokeratins) and predictive (PD-L1) testing. These results emphasize the need for adequate fixation of resection specimen.
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