Evidence map›Paper›PMID 31264021›Full record

ArticleTransgenic research2019

The maize α-zein promoter can be utilized as a strong inducer of cellulase enzyme expression in maize kernels.

Deborah Vicuna Requesens, Maria Elena Gonzalez Romero, Shivakumar P Devaiah, Yeun-Kyung Chang, Ashley Flory, Stephen Streatfield, Rebecca Ring, Cassie Phillips, Nathan C Hood, Cyrus Dean Marbaniang and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Deborah Vicuna RequesensArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
Maria Elena Gonzalez RomeroArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
Shivakumar P DevaiahArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
Yeun-Kyung ChangArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
Ashley FloryArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
Stephen StreatfieldFraunhofer USA Center for Molecular Biotechnology, Newark, DE, 19711, USA.
Rebecca RingArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
Cassie PhillipsArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
Nathan C HoodInfinite Enzymes, LLC, Jonesboro, AR, 72401, USA.
Cyrus Dean MarbaniangArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA.
John A HowardApplied Biotechnology Institute, San Luis Obispo, CA, 93407, USA.
Elizabeth E HoodArkansas State University Biosciences Institute and College of Agriculture, Arkansas State University, State University, AR, 72467, USA. ehood@astate.edu.ORCID 0000-0001-8517-2618
Arkansas State University · USApplied Biotechnology Institute · USFraunhofer USA Center for Molecular Biotechnology · USUniversity of Missouri–St. Louis · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expression of recombinant proteins in plants is a technology for producing vaccines, pharmaceuticals and industrial enzymes. For the past several years, we have produced recombinant proteins in maize kernels using only the embryo, primarily driving expression of foreign genes with the maize globulin-1 promoter. Although strong expression is obtained, these lines use only 10-12% of the seed tissue. If strong embryo expression could be combined with strong endosperm expression, much more recombinant protein could be recovered from a set amount of seed biomass. In this study, we tested three endosperm promoters for expression of a cellulase gene. Promoters tested were rice globulin and glutelin promoters and a maize 19 kDa α-zein promoter. The rice promoters were used in two tandem expression constructs as well. Although the rice promoters were active in producing stable amounts of cellulase, the α-zein promoter was by far the most effective: as much as 9% of total soluble protein was recovered from seed of several independent events and plants. One or two inserts were detected by Southern blot in several lines, indicating that copy number did not appear to be responsible for the differences in protein accumulation. Tissue print analysis indicated that expression was primarily in the endosperm.

Indexed as

CellulaseGene Expression Regulation, PlantGlobulinsGlutensOryzaPlants, Genetically ModifiedPromoter Regions, GeneticSeedsZea maysZeinCellulaseGlobulinsGlutensZeinEndosperm promotersMaizeMaize α zeinRecombinant protein expressionRice globulinRice glutelin

Identifiers

PMID31264021
OpenAlexW2954051593

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.