ArticleACS central science2019
Molecular Basis of the Glucose Transport Mechanism in Plants.
Article in ACS central science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 74 citations in OpenAlex.
- ESMDynamic: Fast and accurate prediction of protein dynamic contact maps from single sequences.Nature communications · 2026Article
- Article
- Tailored for swift recognition: A structural perspective on secondary active plant hormone transporters.The Plant journal : for cell and molecular biology · 2026Review
- Towards a Holistic View of the Orchestration Between Sugar Transporters in Cereal Crops.Plants (Basel, Switzerland) · 2026Article
- Advanced Computational Methods in Protein Simulations: A Case Study of Enhanced Sampling Applied to Membrane Transporters.Advances in experimental medicine and biology · 2026Review
- Stemflow Unveils Trees' Weatherproofing Stratagem During Autumnal Senescence.Plant, cell & environment · 2025Article
- Molecular insights into the elevator-type mechanism of the cyanobacterial bicarbonate transporter BicA.Biophysical journal · 2025Article
- Energetics of substrate transport in proton-dependent oligopeptide transporters.Communications chemistry · 2024Article
- Disruption of the OsWRKY71 transcription factor gene results in early rice seed germination under normal and cold stress conditions.BMC plant biology · 2024Article
- Deep mutational scanning reveals sequence to function constraints for SWEET family transporters.bioRxiv : the preprint server for biology · 2024Article
- SWEET family transporters act as water conducting carrier proteins in plants.bioRxiv : the preprint server for biology · 2024Article
- The Arabidopsis AtSWEET13 transporter discriminates sugars by selective facial and positional substrate recognition.Communications biology · 2024Article
- Interplay between phosphorylation and oligomerization tunes the conformational ensemble of SWEET transporters.bioRxiv : the preprint server for biology · 2024Article
- Functional regulation of aquaporin dynamics by lipid bilayer composition.Nature communications · 2024Article
- Pineapple SWEET10 is a glucose transporter.Horticulture research · 2023Article
- Functional Regulation of Aquaporin Dynamics by Lipid Bilayer Composition.bioRxiv : the preprint server for biology · 2023Article
- Activation mechanism of the human Smoothened receptor.Biophysical journal · 2023Article
- Variations in exons 11 and 12 of the multi-pest resistance wheat geneFrontiers in plant science · 2022Article
- Phylogenetic analysis and structural prediction reveal the potential functional diversity between green algae SWEET transporters.Frontiers in plant science · 2022Article
- Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate sugar transport and storage in tomato fruits.Horticulture research · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SWEET family belongs to a class of transporters in plants that undergoes large conformational changes to facilitate transport of sugar molecules across the cell membrane (SWEET, Sugars Will Eventually Be Exported Transporter). However, the structures of their functionally relevant conformational states in the transport cycle have not been reported. In this study, we have characterized the conformational dynamics and complete transport cycle of glucose in the OsSWEET2b transporter using extensive molecular dynamics simulations. Using Markov state models, we estimated the free energy barrier associated with different states as well as for the glucose transport mechanism. SWEETs undergo a structural transition to outward-facing (OF), occluded (OC), and inward-facing (IF) and strongly support an alternate access transport mechanism. The glucose diffuses freely from outside to inside the cell without causing major conformational changes which means that the conformations of glucose unbound and bound snapshots are exactly the same for OF, OC, and IF states. We identified a network of hydrophobic core residues at the center of the transporter that restricts the glucose entry to the cytoplasmic side and acts as an intracellular hydrophobic gate. The mechanistic predictions from molecular dynamics simulations are validated using site-directed mutagenesis experiments. Our simulation also revealed hourglass-like intermediate states making the pore radius narrower at the center. This work provides new fundamental insights into how substrate-transporter interactions actively change the free energy landscape of the transport cycle to facilitate enhanced transport activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.