ArticleProceedings of the National Academy of Sciences of the United States of America2019
De novo design of symmetric ferredoxins that shuttle electrons in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- The Ascent of Miniproteins.Biochemistry · 2026Review
- The Borderlands of Foldability: Lessons from Simplified Proteins.Trends in chemistry · 2026Article
- Revisiting Eck and Dayhoff's Building Block Model of Ferredoxin Evolution on Dayhoff's 100th Birthday.Journal of molecular evolution · 2026Article
- Article
- On the emergence of metabolism: the evolution of proteins that powered life.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- A Bioelectrochemical Crossbar Architecture Screening Platform (BiCASP) for Extracellular Electron Transfer.bioRxiv : the preprint server for biology · 2025Article
- Understanding the role of negative charge in the scaffold of an artificial enzyme for COJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2024Article
- Ferredoxins: Functions, Evolution, Potential Applications, and Challenges of Subtype Classification.Current issues in molecular biology · 2024Review
- A cellular selection identifies elongated flavodoxins that support electron transfer to sulfite reductase.Protein science : a publication of the Protein Society · 2023Article
- Designed Rubredoxin miniature in a fully artificial electron chain triggered by visible light.Nature communications · 2023Article
- On the potential roles of phosphorus in the early evolution of energy metabolism.Frontiers in microbiology · 2023Review
- Desaturases: Structural and mechanistic insights into the biosynthesis of unsaturated fatty acids.IUBMB life · 2022Review
- Tailorable Tetrahelical Bundles as a Toolkit for Redox Studies.The journal of physical chemistry. B · 2022Article
- Changing the tracks: screening for electron transfer proteins to support hydrogen production.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Article
- Mineral Element Insiders and Outliers Play Crucial Roles in Biological Evolution.Life (Basel, Switzerland) · 2022Article
- Quantifying structural relationships of metal-binding sites suggests origins of biological electron transfer.Science advances · 2022Article
- De novo metalloprotein design.Nature reviews. Chemistry · 2022Article
- Fine-tuning of FeS proteins monitored via pulsed EPR redox potentiometry at Q-band.Biophysical reports · 2021Article
- Towards a Synthetic Biology Toolset for Metallocluster Enzymes in Biosynthetic Pathways: What We Know and What We Need.Molecules (Basel, Switzerland) · 2021Review
- Diversification of Ferredoxins across Living Organisms.Current issues in molecular biology · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A symmetric origin for bacterial ferredoxins was first proposed over 50 y ago, yet, to date, no functional symmetric molecule has been constructed. It is hypothesized that extant proteins have drifted from their symmetric roots via gene duplication followed by mutations. Phylogenetic analyses of extant ferredoxins support the independent evolution of N- and C-terminal sequences, thereby allowing consensus-based design of symmetric 4Fe-4S molecules. All designs bind two [4Fe-4S] clusters and exhibit strongly reducing midpoint potentials ranging from -405 to -515 mV. One of these constructs efficiently shuttles electrons through a designed metabolic pathway in
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