ArticleThe Journal of cell biology1988
Contact formation during fibroblast locomotion: involvement of membrane ruffles and microtubules.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 171 citations in OpenAlex.
- Article
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- The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.The Journal of cell biology · 2019Article
- Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.Frontiers in synaptic neuroscience · 2019Review
- Functional role for stable microtubules in lens fiber cell elongation.Experimental cell research · 2018Article
- Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.Nature communications · 2016Article
- Dense small molecule labeling enables activator-dependent STORM by proximity mapping.Histochemistry and cell biology · 2016Article
- Prolactin Signaling Stimulates Invasion via Na(+)/H(+) Exchanger NHE1 in T47D Human Breast Cancer Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Polarization of excitation light influences molecule counting in single-molecule localization microscopy.Histochemistry and cell biology · 2015Article
- Ena/VASP regulates mDia2-initiated filopodial length, dynamics, and function.Molecular biology of the cell · 2014Article
- The NFL-TBS.40-63 anti-glioblastoma peptide disrupts microtubule and mitochondrial networks in the T98G glioma cell line.PloS one · 2014Article
- Targeting and transport: how microtubules control focal adhesion dynamics.The Journal of cell biology · 2012Review
- Molecular architecture and function of matrix adhesions.Cold Spring Harbor perspectives in biology · 2011Review
- Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography.Nature cell biology · 2010Article
- Development of an optimized protocol for studying the interaction of filamentous bacteriophage with mammalian cells by fluorescence microscopy.Microscopy research and technique · 2010Article
- Article
- Protein 600 is a microtubule/endoplasmic reticulum-associated protein in CNS neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella.The Journal of cell biology · 2008Article
- Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites.Journal of cell science · 2008Article
- B-FABP-expressing radial glial cells: the malignant glioma cell of origin?Neoplasia (New York, N.Y.) · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have correlated the motility of the leading edge of fibroblasts, monitored by phase-contrast cinematography, with the relative distributions of several cytoskeletal elements (vinculin, tubulin, and actin) as well as with the contact patterns determined by interference reflection microscopy. This analysis has revealed the involvement of both ruffles and microspikes, as well as microtubules in the initiation of focal contact formation. Nascent vinculin sites within the leading edge or at its base, taken as primordial cell-substrate contacts, were invariably colocalized with sites that showed a history of transient, prolonged, or cyclic ruffling activity. Extended microspike structures, often preceded the formation of ruffles. Immunofluorescent labeling indicated that some of these primordial contacts were in close apposition to the ends of microtubules that penetrated into the leading edge. By fluorescence and electron microscopy short bundles of actin filaments found at the base of the leading edge were identified as presumptive, primordial contacts. It is concluded that ruffles and microspikes, either independently or in combination, initiate and mark the sites for future contact. Plaque proteins then accumulate (within 10-30 s) at the contract site and, beneath ruffles, induce localized bundling of actin filaments. We propose that all primordial contacts support traction for leading edge protrusion but that only some persist long enough to nucleate stress fiber assembly. Microtubules are postulated as the elements that select, stabilize, and potentiate the formation of these latter, long-lived contacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.