ArticleGenes2019
Identification and Characterization of MYB-bHLH-WD40 Regulatory Complex Members Controlling Anthocyanidin Biosynthesis in Blueberry Fruits Development.
Article in Genes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A meta-analysis and systematic review of plant growth regulator use in blueberry production.Frontiers in plant science · 2025Pooled it
- BrANL2 negatively regulates the anthocyanin biosynthesis by depressing BrPAL, BrC4H, and Br4CL in purple flowering Chinese cabbage (Brassica rapa var. parachinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Molecular Insights intoPlants (Basel, Switzerland) · 2026Article
- Anthocyanin-Driven Dark Phenotypes in Stress Adaptation.Plants (Basel, Switzerland) · 2026Review
- TheFood chemistry. Molecular sciences · 2026Article
- Review
- The Transcription Factors NFYA1 and GBF3 Jointly Regulate CHS2 to Promote Tangeretin Accumulation and Cold Tolerance in Citrus.Plant biotechnology journal · 2026Article
- Environmental dependence of visible anthocyanin accumulation mediated by theFrontiers in plant science · 2026Article
- ATAC-Seq and RNA-Seq Integration Reveals Chromatin Accessibility and Transcriptional Dynamics During Fruit Color Development in Mulberry.International journal of molecular sciences · 2026Article
- Regulatory mechanisms and multidimensional applications of blueberry anthocyanins: a review of current gaps and future directions.Engineering in life sciences · 2026Review
- The Mechanism of TT2-TypePlants (Basel, Switzerland) · 2025Article
- Divergent volatilomes between wild and cultivated strawberries: MYB transcription factors underlie flavor differences.Food chemistry. Molecular sciences · 2025Article
- Integrated Metabolomic and Transcriptomic Analyses of Anthocyanin Synthesis During Fruit Development inBiology · 2025Article
- Three bilberry bHLHs of subgroup IIIf play divergent roles in fruit anthocyanin and flavonoid biosynthesis.Scientific reports · 2025Article
- BrGSTF12, an anthocyanin-related glutathione S-transferase gene, is essential for light-induced anthocyanin accumulation in zicaitai (Brassica Rapa Var. purpuraria).BMC plant biology · 2025Article
- Identification of the bHLH Transcription Factor Family in Orah Mandarin and the Response ofPlants (Basel, Switzerland) · 2025Article
- Transcriptome and Metabolome Analyses Reveal the Mechanism of Color Differences in Pomegranate (Plants (Basel, Switzerland) · 2025Article
- Time-series transcriptome analysis reveals the cascade mechanism of biological processes following the perturbation of the MVA pathway in Salvia miltiorrhiza.Plant molecular biology · 2025Article
- Functional analysis of AP2/ERF family members in flavonoid biosynthesis of wolfberry.Frontiers in plant science · 2025Article
- Enhanced Synthesis of Volatile Compounds by UV-B Irradiation inMetabolites · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anthocyanins is the main representative of flavonoids in blueberry fruits. The anthocyanins biosynthetic pathway has been extensively studied in numerous model plants and fruit crops at biochemical, genetic, and molecular levels. However, the mechanisms by which the MYB transcription factor/basic helix-loop-helix (bHLH) domain protein/WD-repeat (MYB-bHLH-WD40) complexes regulate anthocyanin biosynthesis in blueberry is still limited. In the present study, we identified 11 MYB, 7 bHLH, and 6 WD40 genes in blueberry fruits, using amino acid sequences of homologous MYB-bHLH-WD40 complexes in Arabidopsis, apple, grape, and strawberry. To understand these mechanisms, the expression patterns of MYB-bHLH-WD40 genes were examined and validated using differentially expressed gene (DEG) analysis and quantitative real-time reverse transcription PCR (qRT-PCR), respectively. The expression patterns of MYB-bHLH-WD40 genes positively correlated with anthocyanin accumulation and color development in blueberry fruits. Consistent with the effects of other transcriptional regulators, the VcMYBL1::GFP, VcbHLH1::GFP, and VcWDL2::GFP fusion proteins were only observed in the nucleus. The protein-protein interactions (PPIs) and bimolecular fluorescence complementation (BiFC) assay suggested a possible link between VcbHLHL1 and VcMYBL1. Finally, a model was proposed and discussed for how the expression of the MYB-bHLH-WD40 complexes can promote anthocyanin biosynthesis in blueberry fruits. To our knowledge, this study was the first to evaluate MYB-bHLH-WD40 complexes in blueberry fruits, and it provides a foundation to dissect the function of the mechanism.
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