Evidence map›Paper›PMID 31261667›Full record

ArticleInternational journal of molecular sciences2019

Testis-Specific Isoform of Na/K-ATPase (ATP1A4) Interactome in Raft and Non-Raft Membrane Fractions from Capacitated Bovine Sperm.

Gayathri D Rajamanickam, John P Kastelic, Jacob C Thundathil

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. The Surface Proteome of Bovine Unsexed and Sexed Spermatozoa.Animals : an open access journal from MDPI · 2025
    Article
  4. Article
  5. Article
  6. Testis-Specific Isoform of NaInternational journal of molecular sciences · 2022
    Review
  7. The Non-Gastric HInternational journal of molecular sciences · 2022
    Article
  8. Article
  9. Review
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Gayathri D RajamanickamDepartment of Veterinary Clinical and Diagnostic Services, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada.
John P KastelicDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada.
Jacob C ThundathilDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada. jthundat@ucalgary.ca.
University of Calgary · CA

Funding

Alberta Livestock and Meat Agency 2010F027RNatural Sciences and Engineering Research Council of Canada RGPN-2014-04850
6 · The paper itself

Abstract

The plasma membrane of sperm contains highly dynamic lipid microdomains (rafts), which house signaling proteins with a role in regulating capacitation. We reported that ATP1A4, the testis-specific isoform of Na/K-ATPase, interacted with caveolin-1, Src, epidermal growth factor receptor (EGFR) and extracellular signal-regulated kinases 1/2 (ERK1/2) in raft and non-raft domains of the plasma membrane of bovine sperm during capacitation. The objective of the present study was to use a proteomic approach to characterize the ATP1A4 interactome in rafts and non-rafts from capacitated bovine sperm. The non-raft interactome included hexokinase 1, plakophilin 1, desmoglein 1, 14-3-3 protein ζ/δ, cathepsin D and heat shock protein beta1 proteins exclusively, whereas glutathione

Indexed as

AnimalsAnnexin A2Cathepsin DCattleDesmogleinsGlutathione TransferaseHeat-Shock ProteinsHexokinaseIsoenzymesMaleMembrane MicrodomainsPlakophilinsProtein BindingSodium-Potassium-Exchanging ATPaseSpermatozoaAnnexin A2Cathepsin DDesmogleinsGlutathione TransferaseHeat-Shock ProteinsHexokinaseIsoenzymesPlakophilinsSodium-Potassium-Exchanging ATPasebullcapacitationlipid raftsNa/K-ATPaseproteomics

Identifiers

PMID31261667
PMCPMC6651793
OpenAlexW2953518241

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.