Evidence map›Paper›PMID 31261659›Full record

ArticleInternational journal of molecular sciences2019

Immunization of Rabbits with Recombinant Human Cytomegalovirus Trimeric versus Monomeric gH/gL Protein Elicits Markedly Higher Titers of Antibody and Neutralization Activity.

Xinle Cui, Zhouhong Cao, Shuishu Wang, Michael Flora, Stuart P Adler, Michael A McVoy, Clifford M Snapper

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Xinle CuiDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. xinle.cui@usuhs.edu.
Zhouhong CaoDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Shuishu WangDepartment of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Michael FloraDepartment of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Stuart P AdlerCMV Research Foundation, Richmond, VA 23229, USA.
Michael A McVoyDepartment of Pediatrics, Virginia Commonwealth University, Richmond, VA 23298, USA.
Clifford M SnapperDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Uniformed Services University of the Health Sciences · USOrthopaedic Research of Virginia · USVirginia Commonwealth University · US

Funding

Uniformed Services University of the Health Sciences USUHS Dean's Research and Education Endowment Fund
6 · The paper itself

Abstract

Congenital human cytomegalovirus (HCMV) infection and HCMV infection of immunosuppressed patients cause significant morbidity and mortality, and vaccine development against HCMV is a major public health priority. HCMV envelope glycoproteins gB, gH, and gL, which constitute the core fusion machinery, play critical roles in HCMV fusion and entry into host cells. HCMV gB and gH/gL have been reported to elicit potent neutralizing antibodies. Recently, the gB/gH/gL complex was identified in the envelope of HCMV virions, and 16-50% of the total gH/gL bound to gB, forming the gB/gH/gL complex. These findings make the gB/gH/gL a unique HCMV vaccine candidate. We previously reported the production of HCMV trimeric gB and gH/gL heterodimers, and immunization with a combination of trimeric gB and gH/gL heterodimers elicited strong synergistic HCMV-neutralizing activity. To further improve the immunogenicity of gH/gL, we produced trimeric gH/gL. Rabbits immunized with HCMV trimeric gH/gL induced up to 38-fold higher serum titers of gH/gL-specific IgG relative to HCMV monomeric gH/gL, and elicited ~10-fold higher titers of complement-dependent and complement-independent HCMV-neutralizing activity for both epithelial cells and fibroblasts. HCMV trimeric gH/gL in combination with HCMV trimeric gB would be a novel promising HCMV vaccine candidate that could induce highly potent neutralizing activities.

Indexed as

AnimalsAntibodies, NeutralizingCHO CellsCricetinaeCricetulusCytomegalovirus VaccinesHumansRabbitsVaccines, SyntheticViral Envelope ProteinsAntibodies, NeutralizingCytomegalovirus Vaccinesglycoprotein H, CytomegalovirusUL115 protein, Human herpesvirus 5Vaccines, SyntheticViral Envelope Proteinscongenital infectioncore fusion machineryHCMV gBHCMV gH/gLhematopoietic stem cell transplantationhuman cytomegalovirus (HCMV)neutralizing antibodysolid organ transplantationvaccine

Identifiers

PMID31261659
PMCPMC6651862
OpenAlexW2954703189

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.