ArticleNutrients2019
Manganese Uptake by A549 Cells is Mediated by Both ZIP8 and ZIP14.
Article in Nutrients, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Manganese-Doped Carbon Nanodots as Next-Generation MRI and Fluorescence Imaging Agents.ACS nano · 2026Article
- Manganese: biology, physiology and role in disease.Cell discovery · 2026Review
- Expression of Manganese Transporters ZIP8, ZIP14, and ZnT10 in Brain Barrier Tissues.International journal of molecular sciences · 2024Article
- Consequences of Disturbing Manganese Homeostasis.International journal of molecular sciences · 2023Review
- Plasma non-transferrin-bound iron uptake by the small intestine leads to intestinal injury and intestinal flora dysbiosis in an iron overload mouse model and Caco-2 cells.Science China. Life sciences · 2023Article
- Metal-ion transporter SLC39A8 is required for brain manganese uptake and accumulation.The Journal of biological chemistry · 2023Article
- Ferroptosis-related signature and immune infiltration characterization in acute lung injury/acute respiratory distress syndrome.Respiratory research · 2023Article
- The Regulation of ZIP8 by Dietary Manganese in Mice.International journal of molecular sciences · 2023Article
- Ferroptosis: a new strategy for Chinese herbal medicine treatment of diabetic nephropathy.Frontiers in endocrinology · 2023Review
- Computational Modeling of Macrophage Iron Sequestration during Host Defense against Aspergillus.mSphere · 2022Article
- Critical Role of Zinc Transporter (ZIP8) in Myeloid Innate Immune Cell Function and the Host Response against Bacterial Pneumonia.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- The Potential Roles of Blood-Brain Barrier and Blood-Cerebrospinal Fluid Barrier in Maintaining Brain Manganese Homeostasis.Nutrients · 2021Review
- Generation of a Polyclonal Antibody against the Mouse Metal Transporter ZIP8.Antibodies (Basel, Switzerland) · 2021Article
- Iron and manganese transport in mammalian systems.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Manganese Accumulation in the Brain via Various Transporters and Its Neurotoxicity Mechanisms.Molecules (Basel, Switzerland) · 2020Review
- A holistic view of mammalian (vertebrate) cellular iron uptake.Metallomics : integrated biometal science · 2020Review
- Localization of ZIP14 and ZIP8 in HIBCPP Cells.Brain sciences · 2020Article
- The Functions of ZIP8, ZIP14, and ZnT10 in the Regulation of Systemic Manganese Homeostasis.International journal of molecular sciences · 2020Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The alveolar epithelia of the lungs require manganese (Mn) as an essential nutrient, but also provide an entry route for airborne Mn that can cause neurotoxicity. Transporters involved in Mn uptake by alveolar epithelial cells are unknown. Recently, two members of the Zrt- and Irt-like protein (ZIP) family of metal transporters, ZIP8 and ZIP14, have been identified as crucial Mn importers in vivo. ZIP8 is by far most abundantly expressed in the lungs, whereas ZIP14 expression in the lungs is low compared to other tissues. We hypothesized that Mn uptake by alveolar epithelial cells is primarily mediated by ZIP8. To test our hypothesis, we used A549 cells, a type II alveolar cell line. Mirroring the in vivo situation, A549 cells expressed higher levels of ZIP8 than cell models for the liver, intestines, and kidney. Quantification of ZIP8 and ZIP14 revealed a strong enrichment of ZIP8 over ZIP14 in A549 cells. Using siRNA technology, we identified ZIP8 and ZIP14 as the major transporters mediating Mn uptake by A549 cells. To our surprise, knockdown of either ZIP8 or ZIP14 impaired Mn accumulation to a similar extent, which we traced back to similar amounts of ZIP8 and ZIP14 at the plasma membrane. Our study highlights the importance of both ZIP8 and ZIP14 in Mn metabolism of alveolar epithelial cells.
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