Observational studyPediatric research2020
Experimental support for multidrug resistance transfer potential in the preterm infant gut microbiota.
Observational study in Pediatric research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Influence of probiotics on faecal antibiotic resistome in neonates: a systematic review.Pediatric research · 2026Pooled it
- The prototypic crAssphage is a linear phage-plasmid.Cell host & microbe · 2025Article
- Analysis and culturing of the prototypic crAssphage reveals a phage-plasmid lifestyle.bioRxiv : the preprint server for biology · 2024Article
- Synergistic horizontal transfer of antibiotic resistance genes and transposons in the infant gut microbial genome.mSphere · 2024Article
- Outbreak of OXA-48-producing Enterobacteriaceae in a neonatal intensive care unit in Western Sweden.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Identification of integrons and gene cassette-associated recombination sites in bacteriophage genomes.Frontiers in microbiology · 2023Article
- Molecular epidemiology and comparative genomics of carbapenemase-producingFrontiers in microbiology · 2023Article
- Potential of Aromatic Plant-Derived Essential Oils for the Control of Foodborne Bacteria and Antibiotic Resistance in Animal Production: A Review.Antibiotics (Basel, Switzerland) · 2022Review
- Nasogastric enteral feeding tubes modulate preterm colonization in early life.Pediatric research · 2022Article
- Capturing the antibiotic resistome of preterm infants reveals new benefits of probiotic supplementation.Microbiome · 2022Article
- Functional Dissection of P1 Bacteriophage Holin-like Proteins Reveals the Biological Sense of P1 Lytic System Complexity.International journal of molecular sciences · 2022Article
- Article
- Incompatibility Group I1 (IncI1) Plasmids: Their Genetics, Biology, and Public Health Relevance.Microbiology and molecular biology reviews : MMBR · 2021Review
- Grand Challenges in Understanding Gut Microbes.Frontiers in microbiology · 2021Article
- Mobilome and Resistome Reconstruction from Genomes Belonging to Members of theMicroorganisms · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is currently a lack of experimental evidence for horizontal gene transfer (HGT) mechanisms in the human gut microbiota. The aim of this study was therefore to experimentally determine the HGT potential in the microbiota of a healthy preterm infant twin pair and to evaluate the global occurrence of the mobilized elements.
methodsStool samples were collected. Both shotgun metagenome sequencing and bacterial culturing were done for the same samples. A range of experimental conditions were used to test DNA transfer for the cultured isolates. Searches for global distribution of transferable elements were done for the ~120,000 metagenomic samples in the Sequence Read Archive (SRA) database.
resultsDNA transfer experiments demonstrated frequent transmission of an ESBL encoding IncI1 plasmid, a high copy number ColEI plasmid, and bacteriophage P1. Both IncI1 and ColE1 were abundant in the stool samples. In vitro competition experiments showed that transconjugants containing IncI1 plasmids outcompeted the recipient strain in the absence of antibiotic selection. The SRA searches indicated a global distribution of the mobilizable elements, with chicken identified as a possible reservoir for the IncI1 ESBL encoding plasmid.
conclusionOur results experimentally support a major horizontal transmission and persistence potential of the preterm infant gut microbiota mobilome involving genes encoding ESBL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.