ArticlePLoS biology2019
A polymorphic helix of a Salmonella needle protein relays signals defining distinct steps in type III secretion.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system.Nature communications · 2025Article
- "A high throughput flow cytometry assay for quantifying type 3 secretion system assembly inbioRxiv : the preprint server for biology · 2025Article
- Article
- Virulence-associated type III secretion systems in Gram-negative bacteria.Microbiology (Reading, England) · 2023Review
- Recent insights into type-3 secretion system injectisome structure and mechanism of human enteric pathogens.Current opinion in microbiology · 2023Review
- Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants.Chemical reviews · 2022Review
- Recognition of a translocation motif in the regulator HpaA fromFrontiers in plant science · 2022Article
- Cryo-EM structure of the needle filament tip complex of theProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The type 3 secretion system requires actin polymerization to open translocon pores.PLoS pathogens · 2021Article
- Helical reconstruction ofBiochemistry and biophysics reports · 2021Article
- Structure of the cytoplasmic domain of SctV (SsaV) from the Salmonella SPI-2 injectisome and implications for a pH sensing mechanism.Journal of structural biology · 2021Article
- Substrate-engaged type III secretion system structures reveal gating mechanism for unfolded protein translocation.Nature communications · 2021Article
- TheMicroorganisms · 2021Review
- Pore-forming Esx proteins mediate toxin secretion by Mycobacterium tuberculosis.Nature communications · 2021Article
- Cryoelectron-microscopy structure of the enteropathogenicProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Activation of Shigella flexneri type 3 secretion requires a host-induced conformational change to the translocon pore.PLoS pathogens · 2019Article
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Authors and funding
9 authors at 3 institutions in 4 countries.
Funding
Abstract
Type III protein-secretion machines are essential for the interactions of many pathogenic or symbiotic bacterial species with their respective eukaryotic hosts. The core component of these machines is the injectisome, a multiprotein complex that mediates the selection of substrates, their passage through the bacterial envelope, and ultimately their delivery into eukaryotic target cells. The injectisome is composed of a large cytoplasmic complex or sorting platform, a multiring base embedded in the bacterial envelope, and a needle-like filament that protrudes several nanometers from the bacterial surface and is capped at its distal end by the tip complex. A characteristic feature of these machines is that their activity is stimulated by contact with target host cells. The sensing of target cells, thought to be mediated by the distal tip of the needle filament, generates an activating signal that must be transduced to the secretion machine by the needle filament. Here, through a multidisciplinary approach, including solid-state NMR (SSNMR) and cryo electron microscopy (cryo-EM) analyses, we have identified critical residues of the needle filament protein of a Salmonella Typhimurium type III secretion system that are involved in the regulation of the activity of the secretion machine. We found that mutations in the needle filament protein result in various specific phenotypes associated with different steps in the type III secretion process. More specifically, these studies reveal an important role for a polymorphic helix of the needle filament protein and the residues that line the lumen of its central channel in the control of type III secretion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.